RNA synthetic biology tools have primarily been applied in E. coli; however, many other bacteria are of industrial and clinical significance. Thus, the multicolor fluorogenic aptamer Pepper was evaluated in both Gram-positive and Gram-negative bacteria. Suitable HBC–Pepper dye pairs were identified that give blue, green, or red fluorescence signals in the E. coli, Bacillus subtilis, and Salmonella enterica serovar Typhimurium (S. Typhimurium). Furthermore, we found that different RNA scaffolds have a drastic effect on in vivo fluorescence, which did not correlate with the in vitro folding efficiency. One such scaffold termed DF30–tRNA displays 199-fold greater fluorescence than the Pepper aptamer alone and permits simultaneous dual color imaging in live cells.

中文翻译：

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研究 RNA 支架对不同细菌物种中多色荧光适体辣椒的影响

RNA合成生物学工具主要应用于大肠杆菌；然而，许多其他细菌具有工业和临床意义。因此，在革兰氏阳性和革兰氏阴性细菌中对多色荧光适体 Pepper 进行了评估。确定了合适的 HBC-胡椒染料对，可在大肠杆菌、枯草芽孢杆菌和鼠伤寒沙门氏菌（S. Typhimurium）中发出蓝色、绿色或红色荧光信号。此外，我们发现不同的RNA支架对体内荧光有显着影响，这与体外折叠效率无关。一种名为 DF30-tRNA 的支架显示的荧光比单独的 Pepper 适体强 199 倍，并且允许在活细胞中同时进行双色成像。