Probiotic-based therapies have shown great potential in the prevention and treatment of many diseases by positively regulating intestinal flora homeostasis. However, the efficacy of oral probiotics is severely limited due to the loss of bioactivity, short intestinal retention time, and insufficient therapeutic effect. Here, based on droplet microfluidics, we developed a hydrogel microsphere with colonic targeting and mucoadhesive capabilities as a multifunctional delivery platform, which can be used for co-delivery of probiotics ( Nissle 1917, EcN) and auxiliary molecules (indole-3-propionic acid, IPA), achieving synergistic therapeutic effects. studies shown that the integrated multifunctional microspheres can significantly reduce intestinal inflammation, repair intestinal barrier function, enhance probiotic colonization in the intestine, and modulate disordered intestinal flora, demonstrating enhanced therapeutic effects in a mouse model of colitis. This work reveals that microfluidic-based smart droplet microspheres can provide a versatile platform for advanced microbial therapies.

中文翻译：

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基于微流控的多功能微球，用于增强益生菌和后生元的口服共同递送

基于益生菌的疗法通过积极调节肠道菌群稳态，在预防和治疗许多疾病方面显示出巨大的潜力。然而，口服益生菌由于生物活性丧失、肠道滞留时间短、治疗效果不足，其功效受到严重限制。在这里，基于液滴微流控，我们开发了一种具有结肠靶向和粘膜粘附能力的水凝胶微球作为多功能递送平台，可用于益生菌（Nissle 1917，EcN）和辅助分子（吲哚-3-丙酸）的共同递送、IPA），达到协同治疗效果。研究表明，集成的多功能微球可以显着减少肠道炎症，修复肠道屏障功能，增强益生菌在肠道的定植，调节紊乱的肠道菌群，在小鼠结肠炎模型中显示出增强的治疗效果。这项工作表明，基于微流体的智能液滴微球可以为先进的微生物疗法提供多功能平台。