Mesenchymal stem cell (MSC)-based therapy is an effective strategy for regenerative therapy. However, safety and ease of use are still issues to be overcome in clinical applications. Exosomes are naturally derived nanoparticles containing bioactive molecules, which serve as ideal cell-free therapeutic modalities. However, issues such as delivery, long-term preservation and activity maintenance of exosomes are other problems that limit their application. In this study, we proposed the use of rapid freeze-dry-thaw macroporous hydrogels for the encapsulation of HucMSC-derived exosomes (HucMSC-Exos) combined with an antimicrobial peptide coating. This exosome-encapsulated hyaluronic acid macroporous hydrogel HD-DP7/Exo can achieve long-term storage and transport by lyophilization and can be rapidly redissolved for treatment. After comprehensively comparing the therapeutic effects of HucMSC-Exos and HucMSC-loaded hydrogels, we found that HucMSC-Exos could also effectively regulate fibroblasts, vascular endothelial cells, and macrophages and inhibit myofibroblast-mediated fibrosis, thus promoting tissue regeneration and inhibiting scar formation in a mouse model of deep second-degree burn infection healing. These properties of lyophilized storage and whole-process-repair make HD-DP7/Exo have potential application value and application prospects.

中文翻译：

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外泌体/抗菌肽负载水凝胶伤口敷料通过 miR-21-5p 介导的多种功能促进无疤痕伤口愈合

基于间充质干细胞（MSC）的治疗是再生治疗的有效策略。然而，安全性和易用性仍然是临床应用中需要克服的问题。外泌体是天然衍生的含有生物活性分子的纳米颗粒，可作为理想的无细胞治疗方式。然而，外泌体的递送、长期保存和活性维持等问题是限制其应用的其他问题。在这项研究中，我们提出使用快速冻干解冻大孔水凝胶与抗菌肽涂层相结合来封装 HucMSC 衍生的外泌体 (HucMSC-Exos)。这种外泌体封装的透明质酸大孔水凝胶HD-DP7/Exo可以通过冻干实现长期储存和运输，并可以快速重新溶解进行治疗。综合比较HucMSC-Exos和HucMSC负载水凝胶的治疗效果后，我们发现HucMSC-Exos还可以有效调节成纤维细胞、血管内皮细胞和巨噬细胞，抑制肌成纤维细胞介导的纤维化，从而促进组织再生并抑制疤痕形成。深二度烧伤感染愈合的小鼠模型。这些冻干储存和全过程修复的特性使得HD-DP7/Exo具有潜在的应用价值和应用前景。