Cinnamaldehyde displays strong antifungal activity against fungi such as , but its precise molecular mechanisms of antifungal action remain inadequately understood. In this investigation, we applied chemoproteomics and bioinformatic analysis to unveil the target proteins of cinnamaldehyde in cells. Additionally, our study encompassed the examination of cinnamaldehyde's effects on cell membranes, mitochondrial malate dehydrogenase activity, and intracellular ATP levels in cells. Our findings suggest that malate dehydrogenase could potentially serve as an inhibitory target of cinnamaldehyde in cells. By disrupting the activity of malate dehydrogenase, cinnamaldehyde interferes with the mitochondrial tricarboxylic acid (TCA) cycle, leading to a significant decrease in intracellular ATP levels. Following treatment with cinnamaldehyde at a concentration of 1 MIC, the inhibition rate of MDH activity was 74.90 %, accompanied by an 84.5 % decrease in intracellular ATP content. Furthermore, cinnamaldehyde disrupts cell membrane integrity, resulting in the release of cellular contents and subsequent cell demise. This study endeavors to unveil the molecular-level antifungal mechanism of cinnamaldehyde via a chemoproteomics approach, thereby offering valuable insights for further development and utilization of cinnamaldehyde in preventing and mitigating food spoilage.

中文翻译：

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基于活性的蛋白质分析技术揭示苹果酸脱氢酶是肉桂醛抗黑曲霉的靶蛋白

肉桂醛对真菌等真菌表现出很强的抗真菌活性，但其抗真菌作用的精确分子机制仍不清楚。在这项研究中，我们应用化学蛋白质组学和生物信息学分析来揭示细胞中肉桂醛的靶蛋白。此外，我们的研究还包括检查肉桂醛对细胞膜、线粒体苹果酸脱氢酶活性和细胞内 ATP 水平的影响。我们的研究结果表明，苹果酸脱氢酶可能作为细胞中肉桂醛的抑制靶点。通过破坏苹果酸脱氢酶的活性，肉桂醛干扰线粒体三羧酸 (TCA) 循环，导致细胞内 ATP 水平显着下降。 1 MIC浓度的肉桂醛处理后，MDH活性抑制率为74.90%，同时细胞内ATP含量下降84.5%。此外，肉桂醛会破坏细胞膜的完整性，导致细胞内容物的释放和随后的细胞死亡。本研究致力于通过化学蛋白质组学方法揭示肉桂醛的分子水平抗真菌机制，从而为进一步开发和利用肉桂醛预防和减轻食品腐败提供有价值的见解。