The bacterium Sporosarcina pasteurii is the most commonly used microorganism for Microbial Induced Calcite Precipitation (MICP) due to its high urease activity. To date, no proper fed‐batch cultivation protocol for S. pasteurii has been published, even though this cultivation method has a high potential for reducing costs of producing microbial ureolytic biomass. This study focusses on fed‐batch cultivation of S. pasteurii DSM33. The study distinguishes between limited fed‐batch cultivation and extended batch cultivation. Simply feeding glucose to a S. pasteurii culture does not seem beneficial. However, it was exploited that S. pasteurii is auxotrophic for two vitamins and amino acids. Limited fed‐batch cultivation was accomplished by feeding the necessary vitamins or amino acids to a culture lacking them. Feeding nicotinic acid to a nicotinic acid deprived culture resulted in a 24% increase of the specific urease activity compared to a fed culture without nicotinic acid limitation. Also, extended batch cultivation was explored. Feeding a mixture of glucose and yeast extract results in OD600 of ≈70 at the end of cultivation, which is the highest value published in literature so far. These results have the potential to make MICP applications economically viable.

中文翻译：

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巴氏孢子八菌培养的饲喂策略可更有效地生产用于 MICP 的尿素分解生物质

细菌巴氏孢子八菌由于其高脲酶活性，是微生物诱导方解石沉淀 (MICP) 最常用的微生物。迄今为止，还没有合适的补料分批培养方案巴氏沙门氏菌已发表，尽管这种培养方法具有降低微生物尿素分解生物质生产成本的巨大潜力。本研究的重点是补料分批培养巴氏沙门氏菌帝斯曼33。该研究区分了有限补料分批培养和延长分批培养。只需将葡萄糖喂入巴氏沙门氏菌文化似乎没有什么好处。然而却被利用了巴氏沙门氏菌两种维生素和氨基酸营养缺陷。有限补料分批培养是通过向缺乏维生素或氨基酸的培养物中补料来完成的。与没有烟酸限制的补料培养物相比，向缺乏烟酸的培养物补料烟酸导致比脲酶活性增加24%。此外，还探索了扩大批量培养。饲喂葡萄糖和酵母提取物的混合物导致培养结束时OD600约为70，这​​是迄今为止文献中发表的最高值。这些结果有可能使 MICP 应用在经济上可行。