Hance (AC) is popularly consumed as a kind of medicine homologous food plant. This paper was to examine the hepatoprotective effects and mechanism of AC against liver damage triggered by APAP. Results displayed that AC extract could significantly reduce AST and ALT activities and alleviate histopathological injury via attenuating oxidative stress, hepatocyte apoptosis, and inflammation. , AC extract inhibited the ROS production and apoptosis by inhibiting MAPK signaling pathway in APAP-induced HepG2. Inflammation was relieved via blocking NF-κB signaling pathways in LPS-induced THP-1 cell. Furthermore, we found that AC extract was capable of regulating the expression of APAP metabolism-related enzymes, thus promoting the excretion and detoxification of APAP. Finally, a reliable UPLC-MS/MS method was set up for the chemical profiling of AC extract. These results indicated that AC is expected to be developed as a functional food or health product for the prevention and treatment of APAP poisoning.

中文翻译：

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广州相思子通过抑制 MAPK 和 NF-κB 信号通路减轻 APAP 引发的急性肝损伤

汉斯（AC）作为一种药同源食品植物被广泛食用。本文旨在探讨AC对APAP引起的肝损伤的保肝作用及其机制。结果表明，AC 提取物可以显着降低 AST 和 ALT 活性，通过减轻氧化应激、肝细胞凋亡和炎症来减轻组织病理学损伤。 , AC 提取物通过抑制 APAP 诱导的 HepG2 中的 MAPK 信号通路来抑制 ROS 产生和细胞凋亡。通过阻断 LPS 诱导的 THP-1 细胞中的 NF-κB 信号通路来缓解炎症。此外，我们发现AC提取物能够调节APAP代谢相关酶的表达，从而促进APAP的排泄和解毒。最后，建立了可靠的 UPLC-MS/MS 方法用于 AC 提取物的化学分析。这些结果表明AC有望开发为预防和治疗APAP中毒的功能食品或保健品。