Exposure to bioaerosol contamination has detrimental effects on human health. Recent advances in ATP bioluminescence provide more opportunities for the quantitative detection of bioaerosols. Since almost all active organisms can produce ATP, the amount of airborne microbes can be easily measured by detecting ATP-driven bioluminescence. The accurate evaluation of microorganisms mainly relies on following the four key steps: sampling and enrichment of airborne microbes, lysis for ATP extraction, enzymatic reaction, and measurement of luminescence intensity. To enhance the effectiveness of ATP bioluminescence, each step requires innovative strategies and continuous improvement. In this review, we summarized the recent advances in the quantitative detection of airborne microbes based on ATP bioluminescence, which focuses on the advanced strategies for improving sampling devices combined with ATP bioluminescence. Meanwhile, the optimized and innovative strategies for the remaining three key steps of the ATP bioluminescence assay are highlighted. The aim is to reawaken the prosperity of ATP bioluminescence and promote its wider utilization for efficient, real-time, and accurate detection of airborne microbes.

中文翻译：

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空气采样和 ATP 生物发光定量检测空气中的微生物

暴露于生物气溶胶污染会对人类健康产生不利影响。 ATP 生物发光的最新进展为生物气溶胶的定量检测提供了更多机会。由于几乎所有活性生物体都能产生 ATP，因此通过检测 ATP 驱动的生物发光可以轻松测量空气中微生物的数量。对微生物的准确评价主要依靠以下四个关键步骤：空气中微生物的采样和富集、裂解提取ATP、酶促反应和发光强度测量。为了增强 ATP 生物发光的有效性，每一步都需要创新策略和持续改进。本文综述了基于ATP生物发光的空气微生物定量检测的最新进展，重点介绍了结合ATP生物发光改进采样装置的先进策略。同时，重点介绍了 ATP 生物发光测定其余三个关键步骤的优化和创新策略。其目的是重新唤醒 ATP 生物发光的繁荣，并促进其更广泛地利用，以高效、实时、准确地检测空气中的微生物。