is an herbal plant that belongs to the Campanulaceae family, the dried roots (Dangshen) of which have long been used in traditional Chinese medicine to treat dyspepsia, hypertension, and hyperglycemia. The development of roots is very crucial for producing high-quality Dangshen due to their main medicinal components. However, i the regulation mechanism of root development remains limited. Here, we identified two subunits CpEXO70A1 and CpEXO70A3, of the evolutionarily conserved exocyst complex in . Either or responded to IAA, ABA, and drought treatments. The subcellular localization and GUS-staining assay indicated that CpEXO70A1 and CpEXO70A3 were located in the plasma membrane and exhibited temporal and spatial expression patterns. Overexpression or CRISPR knock-out of the two genes led to reduced biomass in hairy roots, correlating with changes in auxin concentration. Besides, a model predicting the impact of auxin-PIN-EXO70A3 onon root growth and development was proposed. Our study aims to enhance Dangshen yield through molecular breeding in the future.

中文翻译：

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CpEXO70A1/3通过影响中药党参生长素积累调控根系发育

党参是一种属于桔梗科的草本植物，其干燥根（党参）长期以来在中药中用于治疗消化不良、高血压和高血糖。由于其主要药用成分，根部的发育对于生产优质党参至关重要。然而，根发育的调节机制仍然有限。在这里，我们鉴定了 中进化保守的外囊复合体的两个亚基 CpEXO70A1 和 CpEXO70A3。或者对 IAA、ABA 和干旱处理有反应。亚细胞定位和GUS染色分析表明CpEXO70A1和CpEXO70A3位于质膜中并表现出时间和空间表达模式。这两个基因的过度表达或 CRISPR 敲除导致毛状根的生物量减少，与生长素浓度的变化相关。此外，还提出了预测生长素-PIN-EXO70A3对根系生长发育影响的模型。我们的研究旨在未来通过分子育种提高党参产量。