Porcine deltacoronavirus (PDCoV) is a major cause of diarrhea and diarrhea-related deaths among piglets and results in massive losses to the overall porcine industry. The clinical manifestations of porcine diarrhea brought on by the porcine epidemic diarrhea virus (PEDV), porcine transmissible gastroenteritis virus (TGEV), and PDCoV are oddly similar to each other. Hence, the identification of different pathogens through molecular diagnosis and serological techniques is crucial. Three novel detection methods for identifying PDCoV have been developed utilizing recombinase-aided amplification (RAA) or reverse transcription recombinase-aided amplification (RT-RAA) in conjunction with Pyrococcus furiosus Argonaute (PfAgo): RAA-PfAgo, one-pot RT-RAA-PfAgo, and one-pot RT-RAA-PfAgo-LFD. The indicated approaches have a detection limit of around 60 copies/μL of PDCoV and do not cross-react with other viruses including PEDV, TGEV, RVA, PRV, PCV2, or PCV3. The applicability of one-pot RT-RAA-PfAgo and one-pot RT-RAA-PfAgo-LFD were examined using clinical samples and showed a positive rate comparable to the qPCR method. These techniques offer cutting-edge technical assistance for identifying, stopping, and managing PDCoV.

中文翻译：

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基于激烈火球菌 Argonaute 的猪 Delta 冠状病毒检测分析

猪三角洲冠状病毒（PDCoV）是仔猪腹泻和腹泻相关死亡的主要原因，给整个养猪业造成巨大损失。由猪流行性腹泻病毒（PEDV）、猪传染性胃肠炎病毒（TGEV）和PDCoV引起的猪腹泻的临床表现非常相似。因此，通过分子诊断和血清学技术鉴定不同的病原体至关重要。利用重组酶辅助扩增 (RAA) 或逆转录重组酶辅助扩增 (RT-RAA) 与激烈火球菌Argonaute (PfAgo) 相结合，开发了三种用于识别 PDCoV 的新型检测方法：RAA-PfAgo、一锅 RT-RAA -PfAgo 和一锅 RT-RAA-PfAgo-LFD。所示方法的 PDCoV 检测限约为 60 拷贝/μL，并且不会与其他病毒（包括 PEDV、TGEV、RVA、PRV、PCV2 或 PCV3）发生交叉反应。使用临床样本检验一锅法 RT-RAA-PfAgo 和一锅法 RT-RAA-PfAgo-LFD 的适用性，并显示出与 qPCR 方法相当的阳性率。这些技术为识别、阻止和管理 PDCoV 提供了尖端的技术援助。