Summary Environmental disasters like drought reduce agricultural output and plant growth. Redox management significantly affects plant stress responses. An earlier study found that PbPIP1;4 transports H2O2 and promotes H2O2 downstream cascade signaling to restore redox equilibrium. However, this regulatory mechanism requires additional investigation. In this search, the AP2 domain-containing transcription factor was isolated by screening Y1H from the wild pear (Pyrus betulaefolia) cDNA library, named PbERF3. The OE of PbERF3 in pear callus and Arabidopsis enhanced plant resistance to drought and re-established redox balance. The transcripts of the NCEDs gene were upregulated under drought stress. The drought stress-related ABA signaling pathway modulates PbERF3. PbERF3 silencing lowered drought tolerance. Furthermore, yeast 2-hybrid (Y2H), luciferase (LUC), bimolecular fluorescence complementation (BiFC), and co-immunoprecipitation (CO-IP) assays verified that PbERF3 physically interacted with PbHsfC1a. The PbERF3-PbHsfC1a heterodimer coordinately bound to PbPIP1;4 and PbNCED4 promoter, therefore activating both the H2O2 and the ABA signaling pathway. This work revealed a novel PbERF3-PbHsfC1a-PbNCED4-PbPIP1;4 regulatory module, in which PbERF3 interacts with PbHsfC1a to trigger the expression of target genes. This module establishes an interaction between the H2O2 signaling component PbPIP1;4 and the ABA pathways component PbNCED4, enabling a response to drought.

中文翻译：

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Pyrus betulaefolia ERF3 与 HsfC1a 相互作用协调调节水通道蛋白 PIP1;4 和 NCED4 以获得耐旱性

摘要 干旱等环境灾害会降低农业产量和植物生长。氧化还原管理显着影响植物的应激反应。一项早期研究发现，PbPIP1;4 转运 H2O2 并促进 H2O2 下游级联信号传导以恢复氧化还原平衡。然而，这种监管机制需要进一步的研究。本次研究通过从野生梨（Pyrus betulaefolia）cDNA文库中筛选Y1H，分离出含有AP2结构域的转录因子，命名为PbERF3。梨愈伤组织和拟南芥中 PbERF3 的 OE 增强了植物对干旱的抵抗力并重新建立了氧化还原平衡。 NCEDs 基因的转录本在干旱胁迫下上调。干旱胁迫相关的 ABA 信号通路调节 PbERF3。 PbERF3 沉默降低了耐旱性。此外，酵母 2-杂交 (Y2H)、荧光素酶 (LUC)、双分子荧光互补 (BiFC) 和免疫共沉淀 (CO-IP) 测定验证了 PbERF3 与 PbHsfC1a 发生物理相互作用。 PbERF3-PbHsfC1a 异二聚体与 PbPIP1;4 和 PbNCED4 启动子协同结合，从而激活 H2O2 和 ABA 信号通路。这项工作揭示了一种新型的PbERF3-PbHsfC1a-PbNCED4-PbPIP1;4调控模块，其中PbERF3与PbHsfC1a相互作用以触发靶基因的表达。该模块在 H2O2 信号成分 PbPIP1;4 和 ABA 途径成分 PbNCED4 之间建立相互作用，从而能够响应干旱。