Deoxynivalenol (DON), primarily generated by Fusarium species, often exists in agricultural products. It can be transformed to 3-epi-deoxynivalenol (3-epi-DON), with a relatively low toxicity, via two steps. DDH in Pelagibacterium halotolerans ANSP101 was proved to convert DON to 3-keto-deoxynivalenol (3-keto-DON). In the present research, AKR4, a NADPH-dependent aldo/keto reductase from P. halotolerans ANSP101, was identified to be capable of converting 3-keto-DON into 3-epi-DON. Our results demonstrated that AKR4 is clearly a NADPH-dependent enzyme, for its utilization of NADPH is higher than that of NADH. AKR4 functions at a range of pH 5–10 and temperatures of 20–60 °C. AKR4 is able to degrade 89% of 3-keto-DON in 90 min at pH 7 and 50 °C with NADPH as the cofactor. The discovery of AKR4, serving as an enzyme involved in the final step in DON degradation, might provide an option for the final detoxification of DON in food and feed.

中文翻译：

---

NADPH 依赖性醛/酮还原酶负责将耐盐深海杆菌 ANSP101 中的 3-酮-脱氧雪腐镰刀菌烯醇解毒为 3-表-脱氧雪腐镰刀菌烯醇

脱氧雪腐镰刀菌烯醇 (DON) 主要由镰刀菌产生，通常存在于农产品中。它可以通过两步转化为毒性相对较低的3-表-脱氧雪腐镰刀菌烯醇（3-epi-DON）。耐盐远杆菌 ANSP101 中的 DDH 被证明可将 DON 转化为 3-酮-脱氧雪腐镰刀菌烯醇 (3-keto-DON)。在本研究中，AKR4（一种来自 P. halotolerans ANSP101 的 NADPH 依赖性醛/酮还原酶）被鉴定能够将 3-keto-DON 转化为 3-epi-DON。我们的结果表明AKR4显然是一种NADPH依赖性酶，因为它对NADPH的利用率高于NADH。 AKR4 在 pH 5-10 范围和温度 20-60 °C 范围内发挥作用。在 pH 7 和 50 °C 下，以 NADPH 作为辅助因子，AKR4 能够在 90 分钟内降解 89% 的 3-酮-DON。 AKR4 作为参与 DON 降解最后一步的酶，其发现可能为食品和饲料中 DON 的最终解毒提供一种选择。