Besides being scarce, the drugs available for treating cutaneous leishmaniasis have many adverse effects. Ozone is an option to enhance the standard treatment due to the wound-healing activity reported in the literature. In this study, we evaluated the efficiency of ozonated sunflower oil as an adjuvant in treating cutaneous lesions caused by Leishmania amazonensis. BALB/c mice were infected with L. amazonensis, and after the lesions appeared, they were treated in four different schedules using the drug treatment with meglumine antimoniate (Glucantime®), with or without ozonated oil. After thirty days of treatment, the lesions' thickness and their parasitic burden, blood leukocytes, production of NO and cytokines from peritoneal macrophages and lymph node cells were analyzed. The group treated with ozonated oil plus meglumine antimoniate showed the best performance, improving the lesion significantly. The parasitic burden showed that ozonated oil enhanced the leishmanicidal activity of the treatment, eliminating the parasites in the lesion. Besides, a decrease in the TNF levels from peritoneal macrophages and blood leukocytes demonstrated an immunomodulatory action of ozone in the ozonated oil-treated animals compared to the untreated group. Thus, ozonated sunflower oil therapy has been shown as an adjuvant in treating Leishmania lesions since this treatment enhanced the leishmanicidal and wound healing effects of meglumine antimoniate.

除了稀缺之外，可用于治疗皮肤利什曼病的药物还有许多副作用。由于文献报道的伤口愈合活性，臭氧是增强标准治疗的一种选择。在这项研究中，我们评估了臭氧葵花籽油作为佐剂治疗亚马逊利什曼原虫引起的皮肤损伤的功效。 BALB/c 小鼠感染亚马逊乳杆菌，在出现病变后，使用葡甲胺锑酸盐 (Glucantime®) 药物治疗（含或不含臭氧油）按四种不同的方案对它们进行治疗。治疗三十天后，分析病灶的厚度及其寄生负荷、血液白细胞、腹膜巨噬细胞和淋巴结细胞产生的NO和细胞因子。使用臭氧油加葡甲胺锑酸盐治疗的组表现出最好的效果，显着改善了病变。寄生虫负荷表明，臭氧油增强了治疗的杀利什曼活性，消除了病变中的寄生虫。此外，与未处理组相比，经臭氧油处理的动物腹膜巨噬细胞和血液白细胞的 TNF 水平降低，证明臭氧具有免疫调节作用。因此，臭氧化葵花籽油疗法已被证明可以作为治疗利什曼原虫病变的辅助剂，因为这种治疗增强了葡甲胺锑酸盐的杀利什曼病和伤口愈合作用。