This issue of Cytometry Part B, Clinical Cytometry consists of four original articles and four letters to the Editor, bridged by a discussion forum. Although finding common themes between these works is not necessary, some naturally emerged for me under a simple but helpful way of thinking about clinical flow cytometry that I learned from Professor Alberto Orfao's education sessions. To paraphrase, in clinical flow cytometry, we are doing one of three things at any time: identifying, characterizing (as either normal or abnormal) or enumerating cell populations. Fourth, flow cytometry must be interpreted in a broader clinicopathological context. These principles assist in defining the indication and context of use of an assay, which in turn help determine panel design, and other pre-analytical, analytical and post-analytical components. Lastly, this journal recognizes the value of the single case report. While some journals have abandoned them, if well researched, relevant and succinct, they can serve as a useful educational tool or cautionary tale, illustrate the application, strengths or weakness of a guideline, or document rare, interesting cases and other novel phenomena.

Therefore, rather than in order of appearance, I introduce this issue's contents as follows:

本期《细胞计数法 B 部分，临床细胞计数法》包含四篇原创文章和四封致编辑的信，并通过讨论论坛进行衔接。尽管没有必要找到这些作品之间的共同主题，但根据我从 Alberto Orfao 教授的教育课程中学到的一种简单但有用的临床流式细胞术思考方式，一些主题自然而然地浮现出来。换句话说，在临床流式细胞术中，我们随时都在做三件事之一：识别、表征（正常或异常）或计数细胞群。第四，流式细胞术必须在更广泛的临床病理学背景下进行解释。这些原则有助于定义测定的指示和使用背景，进而有助于确定面板设计以及其他分析前、分析和分析后组件。最后，本刊认识到单例报告的价值。虽然一些期刊已经放弃了它们，但如果经过充分研究、相关且简洁，它们可以作为有用的教育工具或警示故事，说明指南的应用、优点或缺点，或记录罕见、有趣的案例和其他新颖现象。

因此，我不按出现顺序，将本期内容介绍如下：