Wounding stress induces the biosynthesis of various secondary metabolites in plants, including anthocyanin. However, the underlying molecular mechanism remains elusive. Here, we reported that a transcription factor, VvWRKY5, promotes wounding-induced anthocyanin accumulation in grape (Vitis vinifera). Biochemical and molecular analyses demonstrated that wounding stress significantly increased anthocyanin content, and VvMYBA1 plays an essential role in this process. VvWRKY5 could interact with VvMYBA1 and amplify the activation effect of VvMYBA1 on its target gene VvUFGT. The transcript level of VvWRKY5 was notably induced by wounding treatment. Moreover, our data demonstrated that VvWRKY5 could promote the synthesis of jasmonic acid (JA), a phytohormone that acts as a positive modulator in anthocyanin accumulation, by directly binding to the W-box element in the promoter of the JA biosynthesis-related gene VvLOX and enhancing its activities, and this activation was greatly enhanced by the VvWRKY5-VvMYBA1 protein complex. Collectively, our findings show that VvWRKY5 plays crucial roles in wounding-induced anthocyanin synthesis in grape and elucidate the transcriptional regulatory mechanism of wounding-induced anthocyanin accumulation.

中文翻译：

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VvWRKY5通过与VvMYBA1相互作用并促进茉莉酸生物合成，正向调节葡萄中受伤引起的花青素积累

创伤应激会诱导植物中各种次生代谢物的生物合成，包括花青素。然而，潜在的分子机制仍然难以捉摸。在这里，我们报道了转录因子 VvWRKY5 促进葡萄（Vitis vinifera）中受伤诱导的花青素积累。生化和分子分析表明，创伤应激显着增加了花青素含量，而VvMYBA1在此过程中发挥着重要作用。 VvWRKY5可以与VvMYBA1相互作用并放大VvMYBA1对其靶基因VvUFGT的激活作用。 VvWRKY5 的转录水平明显受到创伤治疗的诱导。此外，我们的数据表明，VvWRKY5 可以通过直接与 JA 生物合成相关基因 VvLOX 启动子中的 W-box 元件结合，促进茉莉酸（JA）的合成，茉莉酸是一种植物激素，在花青素积累中起到正调节剂的作用。并增强其活性，而 VvWRKY5-VvMYBA1 蛋白复合物大大增强了这种激活作用。总的来说，我们的研究结果表明，VvWRKY5 在葡萄受伤诱导的花青素合成中发挥着至关重要的作用，并阐明了受伤诱导花青素积累的转录调控机制。