A polymer microarray based on the supramolecular ureido-pyrimidinone (UPy) moiety is fabricated to screen antimicrobial materials for their ability to support cell adhesion. UPy-functionalized additives, either cell-adhesive, antimicrobial or control peptides, are used, and investigated in different combinations at different concentrations, resulting in a library of 194 spots. These are characterized on composition and morphology to evaluate the microarray fabrication. Normal human dermal fibroblasts are cultured on the microarrays and cell adhesion to the spots is systematically analyzed. Results demonstrate enhanced cell adhesion on spots with combinations including the antimicrobial peptides. This study clearly proves the power of the high throughput approach in combination with supramolecular molecules, to screen additive libraries for desired biological response.

中文翻译：

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将模块化分子设计与抗菌超分子生物材料上细胞粘附的高通量筛选相结合

制造基于超分子脲基嘧啶酮 (UPy) 部分的聚合物微阵列，用于筛选抗菌材料支持细胞粘附的能力。使用 UPy 功能化添加剂（细胞粘附肽、抗菌肽或对照肽），并以不同浓度的不同组合进行研究，产生了 194 个斑点的库。对它们的组成和形态进行表征，以评估微阵列的制造。在微阵列上培养正常人真皮成纤维细胞，并系统分析细胞对斑点的粘附。结果表明，包含抗菌肽的组合增强了细胞在斑点上的粘附。这项研究清楚地证明了高通量方法与超分子分子相结合的能力，可以筛选添加剂库以获得所需的生物反应。