Overexpression of a gene with unknown function in markedly improved tolerance to lignocellulosic biomass-derived inhibitors. This overexpression also enhanced tolerance to elevated temperatures, ethanol, and high concentrations of NaCl and glucose. Inhibitor degradation and transcriptome analyses related this ultiple tress esistance () gene to the robustness of yeast cells. Nuclear localization and DNA-binding domain analyses indicate that KmMsr is a putative transcriptional regulator. Overexpression of a mutant protein with deletion in the flexible region between amino acids 100 and 150 further enhanced tolerance to multiple inhibitors during fermentation, with ethanol production and productivity increasing by 36.31 % and 80.22 %, respectively. In simultaneous saccharification co-fermentation of corncob without detoxification, expression of with the deleted flexible region improved ethanol production by 5-fold at 42 °C and 2-fold at 37 °C. Overexpression of the mutant provides a strategy for constructing robust lignocellulosic biomass using strains.

中文翻译：

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通过过度表达假定的转录调节因子来增强马克斯克鲁维酵母对玉米芯的同步糖化和共发酵

功能未知的基因的过度表达可显着提高对木质纤维素生物质衍生抑制剂的耐受性。这种过度表达还增强了对高温、乙醇、高浓度氯化钠和葡萄糖的耐受性。抑制剂降解和转录组分析将这种多重抗性（）基因与酵母细胞的稳健性联系起来。核定位和 DNA 结合域分析表明 KmMsr 是一种假定的转录调节因子。在氨基酸100和150之间的灵活区域中缺失的突变蛋白的过表达进一步增强了发酵过程中对多种抑制剂的耐受性，乙醇产量和生产率分别增加了36.31%和80.22%。在不进行解毒的玉米芯同步糖化共发酵中，删除柔性区的表达使乙醇产量在 42°C 下提高了 5 倍，在 37°C 下提高了 2 倍。突变体的过度表达提供了使用菌株构建稳健的木质纤维素生物质的策略。