This study elucidates the mechanism of obesity-related adverse pregnancy outcomes and further investigates the effect of resveratrol on reproductive performance in a short- or long-term HFD-induced obese mouse model. Results show that maternal weight had a significant positive correlation with litter mortality in mice. A long-term HFD increased body weight and litter mortality with decreased expression of uterine cytochrome oxidase 4 (COX4), which was recovered by resveratrol in mice. Moreover, HFD decreased the expression of peroxisome proliferator-activated receptor γ coactivator-1α (PGC-1α), nuclear respiratory factors-1 (Nrf-1), and phosphorylated adenosine 5′-monophosphate (AMP)-activated protein kinase (p-AMPK) and increased the expression of phosphorylated extracellular regulated protein kinases (p-ERK) in the uterus. Resveratrol, a polyphenol that can directly bind to the ERK protein, suppressed the phosphorylation of ERK, increased the expression of p-AMPK, PGC-1α and Nrf-1, and decreased litter mortality in mice.

中文翻译：

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子宫线粒体功能在高脂饮食相关不良妊娠结局中的作用以及白藜芦醇的保护作用

本研究阐明了肥胖相关不良妊娠结局的机制，并进一步研究了白藜芦醇对短期或长期 HFD 诱导的肥胖小鼠模型生殖性能的影响。结果表明，母体体重与小鼠窝死亡率呈显着正相关。长期高脂饮食会增加小鼠的体重和窝死亡率，并导致子宫细胞色素氧化酶 4 (COX4) 的表达降低，白藜芦醇可恢复小鼠的这种表达。此外，HFD 降低了过氧化物酶体增殖物激活受体 γ 共激活因子-1α (PGC-1α)、核呼吸因子-1 (Nrf-1) 和磷酸化腺苷 5′-单磷酸 (AMP) 激活蛋白激酶 (p- AMPK）并增加子宫内磷酸化细胞外调节蛋白激酶（p-ERK）的表达。白藜芦醇是一种多酚，可直接与 ERK 蛋白结合，抑制 ERK 磷酸化，增加 p-AMPK、PGC-1α 和 Nrf-1 的表达，降低小鼠窝死亡率。