One new seco-cyclic phorbol derivative and one new phorbol 12-monoester, named 13-oxo-14-isopropylphorbol (2), 12-angelylphorbol (5), together with four known compounds, named 4a-deoxyphorbol, 13-oxo-14-isopropenylphorbol, 12-isobutyrylphorbol, and phorbol-12-(2-methylbutyrate) were obtained by hydrolyzing croton oil. The structures of the compounds were elucidated by spectroscopic analysis. These compounds showed no cytotoxicity (CC₅₀ > 200 μM). Phorbol-12-monoesters 4–6 showed the weak inhibitory activities on syncytia formation induced by HIV-1IIIB (4, EC₅₀ = 56.05 μM; 5, EC₅₀ = 72.97 μM; 6, EC₅₀ = 52.58 μM). The seco-cyclic phorbol derivatives (2, 3) showed no anti-HIV-1 activities (EC₅₀ > 200 μM), which indicated that chemical cleavage of cyclopropanols might affect anti-HIV-1 activity.

一种新的环环佛波醇衍生物和一种新的佛波醇12-单酯，命名为13-oxo-14-异丙基佛波醇（2），12-当归佛波醇（5），以及四种已知化合物，命名为4a-脱氧佛波醇，13-oxo-14通过巴豆油水解得到-异丙烯基佛波醇、12-异丁酰佛波醇和佛波醇-12-(2-甲基丁酸酯)。通过光谱分析阐明了化合物的结构。这些化合物没有表现出细胞毒性（CC ₅₀ > 200 μM）。 Phorbol-12-monoesters 4 – 6对 HIV-1IIIB 诱导的合胞体形成具有弱抑制活性（4，EC ₅₀ = 56.05 μM；5，EC ₅₀ = 72.97 μM；6，EC ₅₀ = 52.58 μM）。开环佛波醇衍生物( 2 , 3 )没有表现出抗HIV-1活性(EC ₅₀ > 200 μM)，这表明环丙醇的化学裂解可能会影响抗HIV-1活性。