Correction to “Triptolide reverses hypoxia‐induced epithelial‐mesenchymal transition and stem‐like features in pancreatic cancer by NF‐κB downregulation”,International Journal of Cancer

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Correction to “Triptolide reverses hypoxia‐induced epithelial‐mesenchymal transition and stem‐like features in pancreatic cancer by NF‐κB downregulation”
International Journal of Cancer ( IF 6.4 ) Pub Date : 2024-03-23 , DOI: 10.1002/ijc.34924

Liu L, Salnikov AV, Bauer N, et al. Triptolide reverses hypoxia-induced epithelial-mesenchymal transition and stem-like features in pancreatic cancer by NF-κB downregulation. Int J Cancer. 2014;134(10):2489-2503. doi:10.1002/ijc.28583.

Concerns have been raised by a third party about the appearance of Figure 5E, suggesting that the Jagged 1 bands have been cropped and pasted, giving the impression that they were run next to each other on the same gel, whereas the β-Actin loading control bands appear uncropped.

The corresponding author has informed the editors of this error, has provided the original blots underlying this figure, and corrections have been made to Figure 5E as follows:

A white line has been introduced to demarcate the BxPc-3, MIA-PaCa2 and AsPC-1 panels, clearly indicating that the Nanog and SOX2 band pairs have been merged by excluding lanes not relevant to Figure 5E.
In the original assembly of Figure 5E, the labels for the Nanog and SOX2 bands were inadvertently swapped. This has been corrected.
An additional β-Actin control band has been added to the Nanog and SOX2 bands. This decision was made due to the previous incorrect presentation of the Nanog and SOX2 bands under the β-Actin control, which was specific to the Notch 1 and Jagged 1 bands only.
The previous β-Actin control for the Notch 1 and Jagged 1 bands has been replaced with a new β-Actin control band from the same gel segment and has been moved up in the corrected figure below the Notch and Jagged bands. This adjustment was necessary because the previous β-Actin control band, although derived from the same proteins, was not extracted from the same gel segment.

The authors regret this oversight and any inconvenience it may have caused.

Regarding Figure 3B,E, the authors would like to clarify that identical β-Actin controls are shown for the c-Rel bands in Figure 3B and the Twist2 bands in Figure 3E (panels “N”). This is correct as both images are from the same gels/blots, and therefore share a common β-Actin loading control.

The corrected Figure 5E is shown as follows:

中文翻译：

更正“雷公藤甲素通过下调 NF-κB 逆转缺氧诱导的胰腺癌上皮间质转化和干细胞样特征”

刘 L、萨尔尼科夫 AV、鲍尔 N 等。雷公藤甲素通过下调 NF-κB 逆转缺氧诱导的胰腺癌上皮间质转化和干细胞样特征。国际癌症杂志。 2014；134(10):2489-2503。 doi：10.1002/ijc.28583。

第三方对图 5E 的外观提出了担忧，表明锯齿状 1 条带已被裁剪和粘贴，给人的印象是它们在同一凝胶上彼此相邻，而 β-肌动蛋白上样对照条带看起来未裁剪。

通讯作者已将此错误告知编辑，并提供了该图的原始印迹，并对图 5E 进行了如下更正：

引入了一条白线来划分 BxPc-3、MIA-PaCa2 和 AsPC-1 面板，清楚地表明 Nanog 和 SOX2 带对已通过排除与图 5E 不相关的泳道而合并。
在图 5E 的原始组装中，Nanog 和 SOX2 带的标签无意中交换了。此问题已得到纠正。
Nanog 和 SOX2 条带中添加了一个额外的 β-肌动蛋白对照条带。做出这一决定是由于之前在 β-肌动蛋白控制下错误地呈现了 Nanog 和 SOX2 条带，该条带仅特定于 Notch 1 和 Jagged 1 条带。
之前用于 Notch 1 和锯齿状 1 带的 β-肌动蛋白对照已被来自同一凝胶片段的新 β-肌动蛋白对照带所取代，并且已在校正后的图中向上移至 Notch 和锯齿状带下方。这种调整是必要的，因为之前的 β-肌动蛋白对照条带虽然源自相同的蛋白质，但不是从相同的凝胶片段中提取的。

作者对这一疏忽及其可能造成的任何不便表示遗憾。

关于图 3B、E，作者想澄清的是，图 3B 中的 c-Rel 条带和图 3E 中的 Twist2 条带显示了相同的 β-肌动蛋白对照（面板“N”）。这是正确的，因为两个图像都来自相同的凝胶/印迹，因此共享共同的 β-肌动蛋白上样对照。

修正后的图5E如下所示：