The root rot mainly caused by Fusarium solani is a bottleneck in the cultivation of Panax notoginseng. In this study, we reported a gene encoding a plant cell wall structural protein, P. notoginseng proline‐rich protein (PnPRPL1), whose transcription was upregulated by F. solani and induced by some hormone signals. The PnPRPL1 recombinant protein significantly inhibited the growth and conidial germination of the root rot pathogens. Downregulation of PnPRPL1 by RNA interference (RNAi) in P. notoginseng leaves increased the susceptibility to F. solani, whereas overexpression of PnPRPL1 in tobacco (Nicotiana tabacum) enhanced the resistance to F. solani. Compared with wild‐type tobacco, the PnPRPL1‐overexpressing transgenic tobacco had higher reactive oxygen species (ROS)‐scavenging enzyme activities, lower ROS levels, and more lignin and callose deposition. The opposite results were obtained for the P. notoginseng expressing PnPRPL1 RNAi fragments. Furthermore, the PnPRPL1 promoter transcription activity was induced by several plant hormones and multiple stress stimuli. In addition, the transcription factor PnWRKY27 activated the expression of PnPRPL1 by directly binding to the promoter region. Thus, PnPRPL1, which is positively regulated by a WRKY transcription factor, encodes an antimicrobial protein that also mediates ROS homoeostasis and callose/lignin deposition during the response to F. solani infection.

中文翻译：

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富含脯氨酸的蛋白 PRPL1 通过调节活性氧平衡和增强细胞壁屏障来增强三七对茄病镰刀菌的防御

根部腐烂主要是由茄病镰刀菌是培育的瓶颈三七。在这项研究中，我们报道了编码植物细胞壁结构蛋白的基​​因，三七富含脯氨酸的蛋白（PnPRPL1），其转录被上调茄镰刀菌并由一些激素信号诱导。 PnPRPL1重组蛋白显着抑制根腐病菌的生长和分生孢子萌发。下调PnPRPL1通过 RNA 干扰 (RNAi)三七叶子增加了易感性茄镰刀菌，而过度表达PnPRPL1在烟草中（烟草）增强了抵抗力茄镰刀菌。与野生型烟草相比，PnPRPL1‐过度表达的转基因烟草具有更高的活性氧（ROS）清除酶活性、更低的ROS水平以及更多的木质素和胼胝质沉积。获得了相反的结果三七表达PnPRPL1RNAi 片段。此外，PnPRPL1启动子转录活性由多种植物激素和多种胁迫刺激诱导。此外，转录因子 PnWRKY27 激活了PnPRPL1通过直接结合到启动子区域。因此，PnPRPL1受 WRKY 转录因子正向调节，编码一种抗菌蛋白，该蛋白还可在响应过程中介导 ROS 稳态和胼胝质/木质素沉积茄镰刀菌感染。