A new method for rapid determination of the content of selective androgenic receptor modulators (SARMs) andarine, cardarine, ligandrol, ostarine and S-23 in capsules by ¹H- and ¹⁹F-high resolution nuclear magnetic resonance spectroscopy was described and validated. Specificity, linearity, accuracy, precision, detection and quantification limits were considered as validation parameters. Full ¹H-, ¹³C- and ¹⁹F-NMR structural assignment of the SARMs is provided as a tool for self-standing identification without a reference standard. Amounts of 7–15 mg of SARMs/capsule were detected in different products with an intermediate precision of 0.8–1.7% in 4 to 20 minutes of analysis time. The validation results and rapidity of analysis confirm the applicability of the method for large-scale screening. The statistical analysis of the results from ¹⁹F- and ¹H-quantitative NMR showed that both approaches were equally effective, thus expanding the potential use of the methodology to non-fluorinated SARMs. At present, no SARM has been approved for human consumption; however, SARMs are actually used by bodybuilders and recreational athletes, who purchase them even though the risk–benefit ratio of these molecules has not been definitively established.

中文翻译：

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通过高分辨率 19F- 和 1H-NMR 光谱定量测定胶囊中选择性雄激素受体调节剂 (SARM) 的筛选方法

^{描述并验证了一种通过1} H 和¹⁹ F 高分辨率核磁共振波谱快速测定胶囊中选择性雄激素受体调节剂（SARM）andarine、cardarine、ligandrol、ostarine 和 S-23 含量的新方法。特异性、线性、准确度、精密度、检测和定量限被视为验证参数。 SARM 的完整¹ H-、¹³ C- 和¹⁹ F-NMR 结构分配可作为无需参考标准的独立鉴定工具。在 4 至 20 分钟的分析时间内，在不同产品中检测到 7-15 毫克 SARMs/胶囊，中间精度为 0.8-1.7%。验证结果和分析速度证实了该方法对于大规模筛选的适用性。¹⁹ F-和¹ H-定量 NMR结果的统计分析表明，两种方法同样有效，从而将该方法的潜在用途扩展到非氟化 SARM。目前，尚无SARM被批准用于人类消费；然而，SARM 实际上被健美运动员和休闲运动员使用，尽管这些分子的风险效益比尚未明确确定，但他们还是购买了它们。