With the aim of screening more bioactivity metabolites, in this study, two new stilbenes, 5-methoxy-6-(4- methoxystyryl)benzofuran (1) and 6-(4-methoxystyryl)benzofuran-5-ol (2) were isolated from the leaves and stems of Bletilla striata. Their structures were established by their 1D, 2D NMR, and MS spectral data. Interestingly, the anti-SARS-CoV-2 (severe acute respiratory syndrome coronavirus 2) activity revealed that compound 1 exhibited potential anti-SARS-CoV-2 activity with IC₅₀ of 15.2 μM for the delta strain and 22.8 μM for the omicron BA.1 strain; compound 2 also showed potential anti-SARS-CoV-2 activity with IC₅₀ of 10.2 μM for the delta strain and 16.8 μM for the omicron BA.1 strain. These values are lower than that of a positive control (with IC₅₀ of 1.2 μM for the delta strain and 3.4 μM for the omicron BA.1 strain).

为了筛选更多的生物活性代谢物，本研究分离出两种新的二苯乙烯类化合物，5-甲氧基-6-(4-甲氧基苯乙烯基)苯并呋喃 ( 1 ) 和 6-(4-甲氧基苯乙烯基)苯并呋喃-5-醇 ( 2 )取自白芨的叶和茎。它们的结构是通过一维、二维核磁共振和质谱数据确定的。有趣的是，抗 SARS-CoV-2（严重急性呼吸综合征冠状病毒 2）活性表明，化合物1表现出潜在的抗 SARS-CoV-2 活性，对于 delta 株，IC ₅₀为 15.2 μM，对于 omicron BA，IC 50 为 22.8 μM .1 应变；化合物2还表现出潜在的抗 SARS-CoV-2 活性，对于 delta 菌株，IC ₅₀为 10.2 μM，对于 omicron BA.1 菌株，IC 50 为 16.8 μM。这些值低于阳性对照（对于 delta 菌株，IC ₅₀为 1.2 μM，对于 omicron BA.1 菌株，IC 50 为 3.4 μM）。