Viral diseases like yellow fever, dengue, and Zika have an alarming impact on public health. These diseases can be transmitted by Aedes mosquito species, such as Ae. albopictus, which is now found in many countries outside its original range. Xenorhabdus and Photorhabdus spp. are enteric bacterial symbionts of insect-preying nematodes and are known to produce an array of natural products with various activities including larvicidal activity. In this study, the effects of natural products produced by four Xenorhabdus and one Photorhabdus bacteria on the ovipositional behavior of Ae. albopictus mosquitoes were assessed. Utilizing a binary choice assay in insect cages, gravid female mosquitoes were presented with two oviposition cups containing water supplemented with varying concentrations of bacterial supernatants (50–1% concentrations) versus control medium. After 72 h, the eggs deposited on filter papers were counted. The oviposition attractant index (OAI) feature of the bacterial supernatant was evaluated using the number of eggs laid in the cups. Notably, all tested supernatants exhibited concentration-dependent deterrence of oviposition. Xenorhabdus cabanillasii displayed the strongest deterrent effect, inhibiting egg-laying at 50–5% concentrations (OAI: − 0.87 to − 0.35), followed by X. nematophila (50–10%, OAI: − 0.82 to − 0.52). Xenorhabdus szentirmaii, X. doucetiae, and P. kayaii showed significant deterrence at ≥ 20% concentrations. Using promoter exchange mutants generated by the easyPACId approach, fabclavine from X. szentirmaii was identified as the bioactive compound with evident deterrent effects. Such deterrents targeting egg-laying could be valuable for controlling populations by disrupting their breeding in suitable habitats.

黄热病、登革热和寨卡病毒等病毒性疾病对公众健康产生惊人的影响。这些疾病可由伊蚊传播，例如伊蚊。白纹伊蚊现在在其原始分布范围之外的许多国家也有发现。致病杆菌属和发光杆菌属。是食虫线虫的肠道细菌共生体，已知可产生一系列具有多种活性（包括杀幼虫活性）的天然产物。在这项研究中，四种致病杆菌属细菌和一种发光杆菌属细菌产生的天然产物对伊蚊产卵行为的影响。对白纹伊蚊进行了评估。在昆虫笼中利用二元选择测定法，向怀孕的雌性蚊子提供两个产卵杯，其中盛有水，并补充有不同浓度的细菌上清液（50-1％浓度）与对照培养基相比。 72小时后，对沉积在滤纸上的卵进行计数。使用杯中产卵的数量来评估细菌上清液的产卵引诱指数（OAI）特征。值得注意的是，所有测试的上清液均表现出浓度依赖性的产卵抑制作用。Xenorhabdus cabanillasii显示出最强的威慑作用，在 50–5% 浓度下抑制产卵（OAI：− 0.87 至 − 0.35），其次是X. nematophila（50–10%，OAI：− 0.82 至 − 0.52）。Xenorhabdus szentirmaii、X. doucetiae和P. kayaii在 ≥ 20% 浓度下表现出显着的威慑作用。使用通过 easyPACId 方法产生的启动子交换突变体，来自X. szentirmaii的 fabclavine被鉴定为具有明显威慑作用的生物活性化合物。这种针对产卵的威慑措施可能会通过扰乱其在适当栖息地的繁殖来控制种群。