Yeast Rho5 is a small GTPase which mediates the response to nutrient and oxidative stress, and triggers mitophagy and apoptosis. We here studied the rapid translocation of a GFP-tagged Rho5 to mitochondria under such stress conditions by live-cell fluorescence microscopy in the background of strains lacking different mitochondrial outer membrane proteins (MOMP). Fun14, Msp1 and Alo1 were found to be required for efficient recruitment of the GTPase, whereas translocation of Dck1 and Lmo1, the subunits of its dimeric GDP/GTP exchange factor (GEF), remained unaffected. An influence of the voltage-dependent anion channel (VDAC) Por1 on the association of GFP-Rho5 with mitochondria under oxidative stress conditions appeared to be strain-dependent. However, epistasis analyses and bimolecular fluorescence complementation (BiFC) studies indicate a genetic and physical interaction. All four strains lacking a single MOMP were investigated for their effect on mitophagy.

中文翻译：

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小酵母 GTPase Rho5 需要特定的线粒体外膜蛋白在氧化应激下易位并与 VDAC Por1 相互作用

酵母 Rho5 是一种小型 GTP 酶，可介导对营养和氧化应激的反应，并触发线粒体自噬和细胞凋亡。我们在这里通过活细胞荧光显微镜在缺乏不同线粒体外膜蛋白（MOMP）的菌株背景下研究了在这种应激条件下 GFP 标记的 Rho5 向线粒体的快速易位。 Fun14、Msp1 和 Alo1 被发现是有效招募 GTPase 所必需的，而二聚体 GDP/GTP 交换因子 (GEF) 的亚基 Dck1 和 Lmo1 的易位则不受影响。在氧化应激条件下，电压依赖性阴离子通道 (VDAC) Por1 对 GFP-Rho5 与线粒体关联的影响似乎是应变依赖性的。然而，上位分析和双分子荧光互补（BiFC）研究表明遗传和物理相互作用。研究了所有四种缺乏单一 MOMP 的菌株对线粒体自噬的影响。