Both and host-derived exosomes are involved in the pathogenic process of alveolar echinococcosis (AE). Exosomes secrete miRNAs that have regulatory roles in host-pathogen interactions in multiple ways. In the present study, we collected and purified supernatants of cultures, as well as human plasma exosomes. High-throughput sequencing showed the identities of 45 exosomal miRNAs in . The lengths of these miRNAs ranged from 19 to 25 nucleotides (nt), with the majority (n = 18) measuring 22 nt. Notably, emu-let-7-5p emerged as the most abundant among these miRNAs, with a detected count of 33,097 and also length of 22 nt. Nanoparticle tracking analysis (NTA) showed that the concentration of exosomes in the plasma of AE patients was lower compared to that in the healthy individuals. This result suggested that the concentration of plasma exosomes was able to distinguish AE patients from healthy individuals. Using qRT-PCR to assess the relative expression of 10 miRNAs of , we showed that the expression of miR-184-3p was downregulated significantly in the exosomes of plasma from AE patients compared to that in the control group. In summary, this study indicates that AE induces a reduction in the concentration of human plasma exosomes, as well as downregulating miR-184-3p in infected individuals.

中文翻译：

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外泌体衍生的 miRNA 的分子分析显示，肺泡包虫病患者的 miR-184-3p 表达降低，外泌体浓度降低


宿主来源的外泌体均参与肺泡包虫病（AE）的致病过程。外泌体分泌的 miRNA 以多种方式在宿主-病原体相互作用中发挥调节作用。在本研究中，我们收集并纯化了培养物上清液以及人血浆外泌体。高通量测序显示了 45 个外泌体 miRNA 的身份。这些 miRNA 的长度范围为 19 至 25 个核苷酸 (nt)，其中大多数 (n = 18) 长度为 22 nt。值得注意的是，emu-let-7-5p 是这些 miRNA 中最丰富的，检测到的数量为 33,097，长度也为 22 nt。纳米颗粒追踪分析（NTA）显示，AE患者血浆中外泌体的浓度低于健康个体。这一结果表明血浆外泌体的浓度能够区分 AE 患者和健康个体。使用qRT-PCR评估10个miRNA的相对表达，我们发现与对照组相比，AE患者血浆外泌体中miR-184-3p的表达显着下调。总之，这项研究表明 AE 会导致人血浆外泌体浓度降低，并下调感染个体中的 miR-184-3p。