This study investigated the impacts of various culture temperatures and light regimes on growth and biochemical constituents of under carbon-supply and nitrogen-limited conditions to improve oil production in algal cells. Results displayed that under a 30 ℃ and 150 μE/m/s regime, there was a significant increase in biomass, total lipids, and lipid productivity. Specifically, these parameters reached 1.83 g/L, 36.25 %, and 130.73 mg/L/d, respectively. Remarkably, prolonging the photoperiod further enhanced the aforementioned three parameters, reaching peak levels of 1.92 g/L, 41.10 %, and 157.54 mg/L/d, respectively, recorded at a 24/0h photoperiod. Compared with cultures grown under normal conditions, these values displayed increments of 1.21-fold, 74.88 %, and 3.01-fold, respectively. Additionally, under optimal conditions, the soluble sugar content reached 79.72 mg/g, and the biodiesel properties were improved. These findings indicate that moderately increasing temperature, light intensity, and photoperiod could achieve the co-production of biomass, lipids, and sugars in .

中文翻译：

---

富碳限氮条件下培养温度和光照条件对莱茵衣藻生物量和脂质积累的影响

本研究调查了在碳供应和氮限制条件下，不同培养温度和光照条件对生长和生化成分的影响，以提高藻类细胞的产油量。结果显示，在30℃和150μE/m/s条件下，生物量、总脂质和脂质生产率显着增加。具体而言，这些参数分别达到 1.83 g/L、36.25% 和 130.73 mg/L/d。值得注意的是，延长光周期进一步增强了上述三个参数，在 24/0h 光周期下记录的峰值水平分别达到 1.92 g/L、41.10% 和 157.54 mg/L/d。与正常条件下培养的培养物相比，这些值分别显示出1.21倍、74.88%和3.01倍的增量。此外，在最佳条件下，可溶性糖含量达到79.72 mg/g，生物柴油性能得到改善。这些发现表明，适度增加温度、光强度和光周期可以实现生物量、脂质和糖的联产。