A halotolerant consortium between microalgae and methanotrophic bacteria could effectively remediate CH and CO, particularly using saline wastewater sources. Herein, 20Z was demonstrated to form a mutualistic association with sp. HS2 at a salinity level above 3.0%. Co-culture significantly enhanced the growth of both microbes, independent of initial inoculum ratios. Additionally, increased methane provision in enclosed serum bottles led to saturated methane removal. Subsequent analyses suggested nearly an order of magnitude increase in the amount of carbon sequestered in biomass in methane-fed co-cultures, conditions that also maintained a suitable cultural pH suitable for methanotrophic growth. Collectively, these results suggest a robust metabolic coupling between the two microbes and the influence of the factors other than gaseous exchange on the assembled consortium. Therefore, multi-faceted investigations are needed to harness the significant methane removal potential of the identified halotolerant consortium under conditions relevant to real-world operation scenarios.

中文翻译：

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释放协同效应：通过甲基微生物 20Z 和小球藻之间的代谢耦合，利用生物甲烷封存的潜力。 HS2

微藻和甲烷氧化细菌之间的耐盐联合体可以有效修复 CH 和 CO，特别是使用含盐废水源。在此，20Z 被证明与 sp 形成互利关联。 HS2 的盐度水​​平高于 3.0%。共培养显着增强了两种微生物的生长，与初始接种比例无关。此外，封闭血清瓶中增加的甲烷供应导致饱和甲烷去除。随后的分析表明，在添加甲烷的共培养物中，生物量中固存的碳量增加了近一个数量级，同时还保持了适合甲烷氧化生长的合适培养物 pH 值。总的来说，这些结果表明两种微生物之间存在强大的代谢耦合，以及气体交换以外的因素对组装的联合体的影响。因此，需要进行多方面的研究，以在与实际操作场景相关的条件下利用已确定的耐盐联合体的显着甲烷去除潜力。