The biofilm of an engineered strain is limited by slow growth and low yield, resulting in an unsatisfactory ability to resist external stress and promote catalytic efficiency. Here, biofilms used as robust living catalysts were manipulated through dual functionalized gene regulation and carrier modification strategies. The results showed that gene overexpression regulates the autoinducer-2 activity, extracellular polymeric substance content and colony behavior of , and the biofilm yield of D overexpressed strains increased by 79.35 % compared to that of the wild type strains ( < 0.05). In addition, the hydrophilicity of polyurethane fibres modified with potassium dichromate increased significantly, and biofilm adhesion increased by 105.80 %. Finally, the isoquercitrin yield in the catalytic reaction of the biofilm reinforced by the D overexpression strain and the modified carrier was 247.85 % higher than that of the untreated group. Overall, this study has developed engineered strains biofilm with special functions, providing possibilities for catalytic applications.

中文翻译：

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基因调控和载体修饰的耦合将细菌生物膜作为强大的活催化剂

工程菌株的生物膜受到生长缓慢和产量低的限制，导致其抵抗外部压力和提高催化效率的能力不理想。在这里，通过双功能化基因调控和载体修饰策略来操纵用作强大活性催化剂的生物膜。结果表明，基因过表达可调节D过表达菌株的自诱导子2活性、胞外聚合物含量和集落行为，D过表达菌株生物膜产量较野生型菌株增加79.35%（<0.05）。此外，经重铬酸钾改性的聚氨酯纤维的亲水性显着提高，生物膜附着力提高105.80%。最终，D过表达菌株与修饰载体增强的生物膜催化反应中异槲皮苷产率较未处理组提高了247.85%。总体而言，本研究开发了具有特殊功能的工程菌株生物膜，为催化应用提供了可能性。