This study presents a novel method for accelerating the granulation of methanogenic anaerobic granular sludge (AnGS) in an upflow anaerobic sludge blanket (UASB) reactor using solid-phase humin (HM). The results demonstrated that HM-mediated AnGS (HM-AnGS) formed rapidly within 50 days. The increase in particle size, settling velocity and mechanical strength was attributed to the rapid granulation of the HM-AnGS. The maximum methane yield of the HM-AnGS was 5-fold higher than that of the control group. This is consistent with the findings, which showed that HM-AnGS had 3.2–3.4 times more methyl-coenzyme M reductase (Mcr) activity and 2.4–2.9 times more adenosine triphosphate (ATP) than control groups. Molecular analyses indicate that HM most likely accelerated interspecies electron transfer (IET) in HM-AnGS (e.g., from ). Furthermore, the HM-AnGS was effective in recovering energy from actual slaughterhouse wastewater.

中文翻译：

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胡敏素介导的厌氧颗粒污泥的形成/表征和增强的产甲烷性能

本研究提出了一种使用固相胡敏素（HM）在上流式厌氧污泥床（UASB）反应器中加速产甲烷厌氧颗粒污泥（AnGS）颗粒化的新方法。结果表明，HM介导的AnGS（HM-AnGS）在50天内迅速形成。颗粒尺寸、沉降速度和机械强度的增加归因于 HM-AnGS 的快速造粒。 HM-AnGS 的最大甲烷产量是对照组的 5 倍。这与研究结果一致，研究结果表明，与对照组相比，HM-AnGS 的甲基辅酶 M 还原酶 (Mcr) 活性高出 3.2-3.4 倍，三磷酸腺苷 (ATP) 活性高出 2.4-2.9 倍。分子分析表明，HM 最有可能加速 HM-AnGS 中的种间电子转移 (IET)（例如，来自 ）。此外，HM-AnGS 可以有效地从实际屠宰场废水中回收能量。