This study established a lipase (CRL) system to catalyze triolein and ethyl ferulate interesterification. The products were identified, and the binding mode between the substrates and CRL was predicted through molecular docking. Three methods for preparing CRL-AuNPs were proposed and characterized. It was found that the addition of 40 mL of 15 nm gold nanoparticles increased the CRL activity from 3.05 U/mg to 4.75 U/mg, but the hybridization efficiency was only 32.7 %. By using 4 mL of 0.1 mg/mL chloroauric acid, the hybridization efficiency was improved to 50.7 %, but the enzyme activity was sharply decreased. However, when the molar ratio of Mb to HAuCl was 0.2, the hybridization efficiency increased to 71.8 %, and the CRL activity was also enhanced to 5.98 U/mg. Under optimal conditions, the enzyme activity of CRL-AuNPs③ was maintained at 95 % after 6 repetitions and 85.6 % after 30 days at room temperature.

中文翻译：

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CRL-AuNP 增强三油酸甘油酯和阿魏酸乙酯的酯交换性能

本研究建立了脂肪酶（CRL）系统来催化三油酸甘油酯和阿魏酸乙酯酯交换反应。对产物进行鉴定，并通过分子对接预测底物与CRL之间的结合模式。提出并表征了三种制备 CRL-AuNPs 的方法。结果发现，添加40 mL 15 nm金纳米颗粒使CRL活性从3.05 U/mg提高到4.75 U/mg，但杂交效率仅为32.7%。使用4 mL 0.1 mg/mL氯金酸，杂交效率提高至50.7%，但酶活性急剧下降。然而，当Mb与HAuCl的摩尔比为0.2时，杂交效率提高到71.8%，CRL活性也提高到5.98 U/mg。在最佳条件下，CRL-AuNPs③的酶活性在6次重复后保持在95%，在室温下30天后保持在85.6%。