The development of integrated co-production of multiple high-purity carotenoids from microalgal cells holds considerable significance for the valorization of microalgae. In this study, the economical microalga was identified as an accumulator of violaxanthin cycle carotenoids, including violaxanthin, antheraxanthin, and zeaxanthin. Notably, a novel and competent approach for the integrated co-production of violaxanthin cycle carotenoids was explored, encompassing four steps: microalgal cultivation, solvent extraction, octadecylsilyl open-column chromatography, and ethanol precipitation. Under optimal co-production conditions, the purities of the obtained violaxanthin, antheraxanthin, and zeaxanthin all exceeded 92%, with total recovery rates of approximately 51%, 40%, and 60%, respectively. Utilizing nuclear magnetic resonance techniques, the purified violaxanthin, antheraxanthin, and zeaxanthin were identified as all--violaxanthin, all--antheraxanthin, and all--zeaxanthin, respectively. This method held significance for the multiproduct biorefinery of the microalga and carried potential future implications for the violaxanthin cycle carotenoids.

中文翻译：

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海洋微拟球藻的价值用于综合联合生产紫黄质循环类胡萝卜素

利用微藻细胞联合生产多种高纯度类胡萝卜素的开发对于微藻的价值化具有重要意义。在这项研究中，经济型微藻被鉴定为紫黄质循环类胡萝卜素的积累者，包括紫黄质、蒽黄质和玉米黄质。值得注意的是，探索了一种新颖且有效的综合联合生产紫黄质循环类胡萝卜素的方法，包括四个步骤：微藻培养、溶剂萃取、十八烷基甲硅烷基开柱色谱和乙醇沉淀。在最佳联产条件下，所得紫罗兰黄素、炭疽病黄素和玉米黄质的纯度均超过92%，总回收率分别约为51%、40%和60%。利用核磁共振技术，纯化的紫罗兰黄质、蒽醌黄质和玉米黄质分别被鉴定为全紫黄质、全炭疽黄质和全玉米黄质。该方法对于微藻的多产品生物精炼具有重要意义，并对紫黄素循环类胡萝卜素具有潜在的未来影响。