HPLC-MS analysis revealed the presence of an unreported peptide in the extract of the marine sponge Neopetrosia sp. Its structure was determined as a tripeptide, named neopetromin (1), composed of two tyrosine and one tryptophan residues with a heteroaromatic C–N cross-link between side chains. The absolute configuration of amino acids was determined using Marfey’s method after ozonolysis and hydrolysis of 1. Compound 1 promoted vacuole fragmentation in an actin-independent manner in tobacco BY-2 cells.

中文翻译：

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Neopetromin 是一种具有 C-N 交联的环状三肽，来自海洋海绵 Neopetrosia sp.，可导致烟草 BY-2 细胞中的液泡破碎

HPLC-MS 分析表明，海绵Neopetrosia sp. 的提取物中存在未报告的肽。其结构被确定为三肽，命名为 Neopetromin ( 1 )，由两个酪氨酸和一个色氨酸残基组成，侧链之间具有杂芳族 C-N 交联。1的臭氧分解和水解后使用Marfey's 方法测定氨基酸的绝对构型。化合物1以不依赖于肌动蛋白的方式促进烟草 BY-2 细胞中的液泡破碎。