The success of contemporary disease management strategies in shrimp aquaculture, such as the ‘systems’ approach, is predicated on robust knowledge of the conditions and interactions between the host, pathogen and environment that promote disease. Pathogen challenge experiments (PCEs) are a power tool for investigating these conditions and interactions. However, absence of accurately reported experimental detail in published PCEs limits scientific transparency, reproducibility, and the potential for the research to make progressive advancements contributing to contemporary shrimp disease management strategies. This review identifies and discusses key factors relating to the host (shrimp), pathogen (virus), and environment that should be carefully considered during the design and publication of PCEs. We offer substantial evidence of their impact on viral disease outcomes, drawn from the existing body of literature, to supporting their consideration. The prevalence of reported experimental details for these factors across 186 viral PCEs in shrimp were evaluated. The review highlights a concerning paucity of experimental detail reported in published shrimp PCEs. We propose a checklist for the minimum reportable information in the publication of shrimp viral PCEs, hereafter referred to as the Shrimp PCE Reporting Guidelines (SPERG). The guidelines aim to enhance the transparency and standardisation of reporting in published PCEs, ensuring that key factors pertaining to the shrimp, pathogen, and environment are adequately considered and documented. Adoption of SPERG is envisaged to empower researchers, reviewers, and readers to assess the internal and external validity of PCEs, facilitating critical evaluation and improved utility of PCE findings for contemporary disease management.

中文翻译：

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迈向虾病毒攻击实验的“系统”方法：出版报告指南

现代对虾养殖疾病管理策略（例如“系统”方法）的成功取决于对促进疾病的宿主、病原体和环境之间的条件和相互作用的深入了解。病原体挑战实验 (PCE) 是研究这些条件和相互作用的有力工具。然而，已发表的 PCE 中缺乏准确报告的实验细节限制了科学透明度、可重复性以及研究为当代虾病管理策略做出贡献的潜力。本综述确定并讨论了在 PCE 的设计和发布过程中应仔细考虑的与宿主（虾）、病原体（病毒）和环境相关的关键因素。我们从现有文献中提取了大量证据，证明它们对病毒性疾病结果的影响，以支持他们的考虑。评估了虾中 186 个病毒 PCE 中报告的这些因素的实验细节的普遍性。该评论强调了已发表的虾 PCE 中报告的实验细节缺乏的问题。我们提出了虾病毒 PCE 出版中最低可报告信息的清单，以下称为虾 PCE 报告指南 (SPERG)。该指南旨在提高已发布的PCE报告的透明度和标准化，确保与虾、病原体和环境有关的关键因素得到充分考虑和记录。SPERG 的采用旨在使研究人员、审稿人和读者能够评估 PCE 的内部和外部有效性，促进关键评估并提高 PCE 研究结果在当代疾病管理中的实用性。