Two Legionella-like isolates, 8cVS16T and 9fVS26, were isolated from a water distribution system (WDS) in a healthcare facility. Cells were Gram- and Ziehl Neelsen-stain-negative, rod-shaped, motile, and exhibited a blue-white fluorescence under Wood’s lamp at 365 nm. The strains grew in a range of 32–37 °C on BCYE with L-cysteine (Cys+), GVPC, and MWY agar medium, with a positive reaction for oxidase, catalase, and gelatinase. The dominant fatty acids were summed features 3 (C16:1ω7c/C16:1ω6c) (27.7%), C16:0 iso (17.5%), and C16:0 (16.3%), and Q13 as the major ubiquinone. The mip and rpoB gene sequences showed a similarity of 96.7% and 92.4%, with L. anisa (ATCC 35292T). The whole genomes sequencing (WGS) performed displayed a GC content of 38.21 mol% for both. The digital DNA-DNA hybridization (dDDH) analysis demonstrated the separation of the two strains from the phylogenetically most related L. anisa (ATCC 35292T), with ≤43% DNA-DNA relatedness. The Average Nucleotide Identity (ANI) between the two strains and L. anisa (ATCC 35292T) was 90.74%, confirming that the two isolates represent a novel species of the genus Legionella. The name proposed for this species is Legionella resiliens sp. nov., with 8cVS16T (=DSM 114356T = CCUG 76627T) as the type strain.

中文翻译：

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从医疗机构中分离出的军团菌新种的特征：弹性军团菌。十一月

从医疗机构的配水系统 (WDS) 中分离出两种类军团菌分离株 8cVS16T 和 9fVS26。细胞为革兰氏染色和齐尼尔森染色阴性、棒状、能动，并在伍德灯下365 nm处呈现蓝白色荧光。菌株在含有 L-半胱氨酸 (Cys+)、GVPC 和 MWY 琼脂培养基的 BCYE 上于 32-37 °C 范围内生长，氧化酶、过氧化氢酶和明胶酶呈阳性反应。主要脂肪酸为特征 3 (C16:1ω7c/C16:1ω6c) (27.7%)、C16:0 iso (17.5%) 和 C16:0 (16.3%)，Q13 为主要泛醌。mip 和 rpoB 基因序列与 L. anisa (ATCC 35292T) 的相似性分别为 96.7% 和 92.4%。进行的全基因组测序 (WGS) 显示两者的 GC 含量均为 38.21 mol%。数字 DNA-DNA 杂交 (dDDH) 分析表明，这两个菌株与系统发育最相关的 L. anisa (ATCC 35292T) 是分离的，DNA-DNA 相关性≤43%。两个菌株与 L. anisa (ATCC 35292T) 之间的平均核苷酸同一性 (ANI) 为 90.74%，证实这两个分离株代表军团菌属的新种。该物种的建议名称为 Legionella resiliens sp。11 月，以 8cVS16T (=DSM 114356T = CCUG 76627T) 作为模式菌株。