Malachite Green (MG) is a widely used industrial dye that is hazardous to health. Herein, the decolourisation and detoxification of MG were achieved using the engineered expressing novel thermostable laccase lcc1 from . The engineered strain RCL produced a high laccase activity of 121.83 U L. Lcc1 was stable at temperatures ranging from 20 ℃ to 60 ℃ and showed a high tolerance to organic solvents. Moreover, Lcc1 could decolorize different kinds of dyes (azo, anthraquinone and triphenylmethane), among which, the decolorization ability of MG is the highest, reaching 95.10 %, and the decolorization rate of other triphenylmethane dyes also over 50 %. The RCL decolorized about 95 % of 50 mg L of MG dye in 10 h at 30 ℃. The MG degradation products were analyzed. The industrial application potential of the RCL was evaluated by treating industrial wastewater and the decolourisation rates were over 90 %.

中文翻译：

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表达来自 Trametes trogii 的新型耐热漆酶的工程酿酒酵母对孔雀石绿的脱色和解毒

孔雀石绿（MG）是一种广泛使用的工业染料，对健康有害。在此，使用工程表达的新型耐热漆酶 lcc1 实现了 MG 的脱色和解毒。工程菌株RCL产生了121.83 U L的高漆酶活性。Lcc1在20 ℃至60 ℃的温度范围内稳定，并且对有机溶剂表现出较高的耐受性。此外，Lcc1可以对多种染料（偶氮、蒽醌和三苯甲烷）进行脱色，其中MG的脱色能力最高，达到95.10%，其他三苯甲烷染料的脱色率也超过50%。 RCL 在 30 ℃ 下 10 h 内对 50 mg/L MG 染料脱色约 95%。分析了 MG 降解产物。通过处理工业废水评价了RCL的工业应用潜力，脱色率超过90%。