In this work, a novel biofilm-based fermentation of was employed to convert -2- phenoxypropionic acid (-PPA) to -2-(4-hydroxyphenoxy) propionic acid (-HPPA). The biofilm culture model of produced a significantly higher -HPPA titer than the traditional submerged fermentation method. Mannitol dosage, tryptone dosage, and initial pH were the factors that played a significant role in biofilm formation and -HPPA synthesis. Under the optimal conditions, the maximum -HPPA titer and productivity approached 22.2 g/L and 3.2 g/(L·d), respectively. A two-stage bioreactor combining agitation and static incubation was developed to further increase -HPPA production. The process was optimized to achieve 100 % conversion of -PPA, with a maximum -HPPA titer of 50 g/L and productivity of 3.8 g/(L·d). This newly developed biofilm-based two-stage fermentation process provides a promising strategy for the industrial production of -HPPA and related hydroxylated aromatic compounds.

中文翻译：

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利用基于生物膜的两阶段发酵白僵菌高效生产 R-2-(4-羟基苯氧基)丙酸

在这项工作中，采用一种新型的基于生物膜的发酵将-2-苯氧基丙酸（-PPA）转化为-2-（4-羟基苯氧基）丙酸（-HPPA）。生物膜培养模型产生的-HPPA滴度明显高于传统深层发酵方法。甘露醇用量、胰蛋白胨用量和初始pH是在生物膜形成和-HPPA合成中起重要作用的因素。在最佳条件下，最大-HPPA滴度和产率分别接近22.2 g/L和3.2 g/(L·d)。开发了结合搅拌和静态孵育的两阶段生物反应器以进一步提高-HPPA产量。该工艺经过优化，可实现-PPA 100% 转化，最大-HPPA 滴度为 50 g/L，生产率为 3.8 g/(L·d)。这种新开发的基于生物膜的两阶段发酵工艺为-HPPA和相关羟基化芳香族化合物的工业生产提供了一种有前景的策略。