The consumption of dietary fiber is beneficial for gut health, but the role of bound polyphenols in dietary fiber has lacked systematic study. The aim of this study is to evaluate the ameliorative effect of mung bean coat dietary fiber (MDF) on DSS-induced ulcerative colitis in mice in the presence and absence of bound polyphenols. Compared to polyphenol-removed MDF (PR-MDF), MDF and formulated-MDF (F-MDF,backfilling polyphenols by the amount of extracted from MDF into PR-MDF) alleviated symptoms such as weight loss and colonic injury in mice with colitis, effectively reduced excessive inflammatory responses, and the bound polyphenols restored the integrity of the intestinal barrier by promoting the expression of tight junction proteins. Additionally, bound polyphenols restored the expression of autophagy-related proteins (mTOR, beclin-1, Atg5 and Atg7) and inhibited the excessive expression of apoptotic-related proteins (Bax, caspase-9, and caspase-3). Furthermore, bound polyphenols could ameliorate the dysregulation of the intestinal microbiota by increasing the abundance of beneficial bacteria and inhibiting the abundance of harmful bacteria. Thus, it can be concluded that the presence of bound polyphenols in MDF plays a key role in the alleviation of DSS-induced ulcerative colitis.

中文翻译：

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绿豆皮膳食纤维中结合多酚对 DSS 诱导的小鼠溃疡性结肠炎的改善作用：肠道屏障和肠道菌群

食用膳食纤维有利于肠道健康，但膳食纤维中结合多酚的作用缺乏系统研究。本研究的目的是评估绿豆皮膳食纤维 (MDF) 在存在和不存在结合多酚的情况下对 DSS 诱导的小鼠溃疡性结肠炎的改善作用。与去多酚中密度纤维板（PR-MDF）相比，中密度纤维板和配方中密度纤维板（F-MDF，将中密度纤维板中提取的多酚量回填到PR-MDF中）减轻了结肠炎小鼠的体重减轻和结肠损伤等症状，有效减少过度炎症反应，结合的多酚通过促进紧密连接蛋白的表达恢复肠道屏障的完整性。此外，结合的多酚恢复了自噬相关蛋白（mTOR、beclin-1、Atg5 和 Atg7）的表达，并抑制了凋亡相关蛋白（Bax、caspase-9 和 caspase-3）的过度表达。此外，结合多酚可以通过增加有益细菌的丰度并抑制有害细菌的丰度来改善肠道微生物群的失调。因此，可以得出结论，MDF 中结合多酚的存在在缓解 DSS 诱导的溃疡性结肠炎中起着关键作用。