The potential of hydrolytic enzyme cocktail obtained from designed bacterial consortium WSh-1 comprising CRN 16, CRN 18, CRN 24, CRN 29, and sp. CRN 30, was investigated for maximum saccharification. Activity was further enhanced to 1.01 U/ml from 0.82 U/ml by supplementing growth medium with biotin and cellobiose as a cofactor and inducer. Through kinetic analysis, the enzyme cocktail showed a high wheat straw affinity with Michaelis-Menten constant (Km) of 0.68 µmol/L and a deconstruction rate (Vmax) of 4.5 U/ml/min. The statistical optimization of critical parameters increased saccharification to 89 %. The optimized process in a 5-L lab-scale bioreactor yielded 501 mg/g of reducing sugar from NaOH-pretreated wheat straw. Lastly, genomic insights revealed unique abundant oligosaccharide deconstruction enzymes with the most diverse CAZyme profile. The consortium-mediated enzyme cocktails offer broader versatility with efficiency for the economical and sustainable valorization of lignocellulosic waste.

中文翻译：

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定制的微生物群落生产水解酶混合物以最大程度地糖化麦秆

从包含 CRN 16、CRN 18、CRN 24、CRN 29 和 sp 的设计细菌群落 WSh-1 获得的水解酶混合物的潜力。研究了 CRN 30 的最大糖化作用。通过在生长培养基中添加生物素和纤维二糖作为辅助因子和诱导剂，活性从 0.82 U/ml 进一步增强至 1.01 U/ml。通过动力学分析，酶混合物对麦秆具有较高的亲和力，米氏常数（Km）为0.68 µmol/L，解构速率（Vmax）为4.5 U/ml/min。关键参数的统计优化将糖化率提高到 89%。在 5 L 实验室规模生物反应器中优化的工艺从 NaOH 预处理的麦秆中产生了 501 mg/g 的还原糖。最后，基因组见解揭示了独特丰富的寡糖解构酶和最多样化的 CAZyme 谱。联合体介导的酶混合物提供了更广泛的多功能性和效率，可实现木质纤维素废物的经济和可持续的增值。