Boosting CO biotransformation by renewable electrical energy provides a prospective avenue to reform the biomanufacturing paradigm and achieve a circular carbon economy. However, mismatches between electrocatalytic and biosynthetic modules lead to poor efficiency. Herein, we design an integrated electro-driven CO-single cell protein (SCP) transformation system, via coupling an electrocatalytic CO reduction to formate module with a formate assimilation module. A Cu/CuO electrocatalyst has been rationally designed with excellent duration stability over 100 h under −0.78 V. Strain adaptability investigation and equipment upgrading are carried out to guarantee the effective integration. SCP has been accumulated to a OD of 4 with an exponential growth rate of 0.114 OD h and electrical energy conversion efficiency of 9.2%. No risky hydrogen is needed and the produced formate is ready-to-use for biosynthesis. The elaborate construction bridges non-biological and biological carbon fixation units and offers a promising blueprint for electro-driven CO biotransformation and scalable bio-manufacturing.

中文翻译：

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通过集成电催化生物合成系统将二氧化碳转化为单细胞蛋白质

利用可再生电能促进二氧化碳生物转化为改革生物制造范式和实现循环碳经济提供了一条有前景的途径。然而，电催化和生物合成模块之间的不匹配导致效率低下。在此，我们通过将电催化CO还原至甲酸盐模块与甲酸盐同化模块耦合，设计了一种集成的电驱动CO-单细胞蛋白质（SCP）转化系统。合理设计了Cu/CuO电催化剂，在-0.78 V下具有100 h以上的优异持续稳定性。并进行了应变适应性研究和设备升级，以保证有效集成。 SCP已累积至OD为4，指数增长率为0.114 OD·h，电能转换效率为9.2%。不需要有风险的氢气，并且产生的甲酸盐可立即用于生物合成。这种精心设计的结构连接了非生物和生物碳固定装置，并为电驱动二氧化碳生物转化和可扩展的生物制造提供了有前景的蓝图。