is a nematophagous fungus which has shown promising results as a non-chemical parasitic control tool. The fungus disrupts the parasite's life cycle by trapping larvae in the environment through the networks generated from chlamydospores, thus preventing the reinfection of animals. One barrier to the development of a commercial product using this tool is the need to increase chlamydospore production in the laboratory for its administration to livestock. The purpose of this study was to evaluate the addition of mannitol to an enriched culture medium and the effect of adverse cultivation conditions on chlamydospore production. was cultivated on Petri dishes with corn agar for 4 weeks at 27 °C and 70% relative humidity (RH). Four groups were then formed, all with Sabouraud agar as a base, to which different growth inducers were added: GSA (glucose Sabouraud agar), GSA-MI (glucose Sabouraud agar + meso inositol), GSA-E (enriched glucose Sabouraud agar), and AE-M (enriched agar + mannitol). After 4 weeks, chlamydospores were recovered by washing the surface of each plate with distilled water and then quantified. The medium that yielded the highest amount of chlamydospores was subjected to different cultivation conditions: NC (normal conditions): 70% RH and 27 °C, AC (adverse conditions) 1: 20% RH and 40 °C, CA2: 60% RH and 27 °C, and CA3: 55% RH and 24 °C. It was determined that mannitol increases chlamydospore production (65x10 chlamydospores/plate), and when reducing humidity by 10% under cultivation conditions it resulted in an approximately 10% increase in chlamydospore production compared to the control group. These results suggest that the addition of polyols, as well as its cultivation under certain environmental conditions, can improve chlamydospore production on a laboratory scale.

中文翻译：

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提高 Duddingtonia flagrans 在固体基质上厚垣孢子的产量：甘露醇和不同培养条件的影响


是一种食线虫真菌，作为非化学寄生虫控制工具已显示出有希望的结果。这种真菌通过厚垣孢子产生的网络将幼虫捕获在环境中，从而破坏寄生虫的生命周期，从而防止动物再次感染。使用该工具开发商业产品的一个障碍是需要增加实验室中厚垣孢子的产量以用于对牲畜的管理。本研究的目的是评估在富集培养基中添加甘露醇以及不利培养条件对厚垣孢子产生的影响。在 27°C 和 70% 相对湿度 (RH) 下，在装有玉米琼脂的培养皿中培养 4 周。然后形成四组，均以沙氏琼脂为基础，添加不同的生长诱导剂：GSA（葡萄糖沙氏琼脂）、GSA-MI（葡萄糖沙氏琼脂+内消旋肌醇）、GSA-E（富集葡萄糖沙氏琼脂）和 AE-M（富集琼脂 + 甘露醇）。 4周后，通过用蒸馏水洗涤每个板的表面来回收厚垣孢子，然后进行定量。厚垣孢子产量最高的培养基接受不同的培养条件：NC（正常条件）：70% RH 和 27 °C，AC（不利条件）1：20% RH 和 40 °C，CA2：60% RH和 27°C，以及 CA3：55% RH 和 24°C。据确定，甘露醇可增加厚垣孢子产量（65x10 厚垣孢子/板），并且当在培养条件下将湿度降低 10% 时，与对照组相比，厚垣孢子产量增加约 10%。 这些结果表明，多元醇的添加以及在某些环境条件下的培养可以提高实验室规模的厚垣孢子产量。