Attention-deficit/hyperactivity disorder (ADHD) lacks objective diagnostic markers. In clinical settings, reliance on subjective judgments can often lead to missed or misdiagnoses. Some biomarkers have been reported to be associated with ADHD, but using one biomarker alone is not enough. To address this, we developed a fluorescent immunoassay platform based on quantum dots (QDs) to detect assay capable of detecting and quantifying multiple biomarkers simultaneously. Specifically, we were able to the simultaneously detect brain-derived neurotrophic factor, tumor necrosis factor-alpha, interleukin-6 and ferritin using different emission spectra QDs. The QD-based multiplexed immunoassay displayed a low detection of limit in the range of 0.021−0.068 pg/mL, and the assay showed satisfactory reproducibility and precision. We then quantified all four targets from ADHD patient’s plasma samples, where it showed remarkable consistency with clinical test for ADHD diagnosis. This methodological comparison supports the diagnosis of ADHD using our assay.

中文翻译：

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基于量子点的多重免疫传感器用于准确诊断儿童注意力缺陷多动障碍

注意力缺陷/多动障碍（ADHD）缺乏客观的诊断标志物。在临床环境中，依赖主观判断常常会导致漏诊或误诊。据报道，一些生物标志物与多动症有关，但仅使用一种生物标志物是不够的。为了解决这个问题，我们开发了一种基于量子点 (QD) 的荧光免疫分析平台，以检测能够同时检测和量化多个生物标志物的分析方法。具体来说，我们能够使用不同的发射光谱量子点同时检测脑源性神经营养因子、肿瘤坏死因子-α、白介素-6 和铁蛋白。基于 QD 的多重免疫分析显示出 0.021−0.068 pg/mL 范围内的低检出限，并且该分析显示出令人满意的重现性和精密度。然后，我们对 ADHD 患者血浆样本中的所有四个目标进行了量化，结果显示与 ADHD 诊断的临床测试具有显着的一致性。这种方法学比较支持使用我们的检测方法诊断 ADHD。