Pre- and/or post-natal administrations of di(2-ethylhexyl) phthalate (DEHP) in experimental animals cause alterations in the spermatogenesis. However, the mechanism by which DEHP affects fertility is unknown and could be through alterations in the survival and differentiation of the gonocytes. The aim of the present study was to evaluate the effect of a single administration of DEHP in newborn mice on gonocytic proliferation, differentiation and survival and its long-term effects on seminiferous epithelium and sperm quality. BALB/c mice distributed into Control and DEHP groups were used. Each animal in the DEHP group was given a single dose of 500 mg/Kg at birth. The animals were analyzed at 1, 2, 4, 6, 8, 10 and 70 days postpartum (dpp). Testicular tissues were processed for morphological analysis to determine the different types of gonocytes, differentiation index, seminiferous epithelial alterations, and immunoreactivity to Stra8, Pcna and Vimentin proteins. Long-term evaluation of the seminiferous epithelium and sperm quality were carried out at 70 dpp. The DEHP animal group presented gonocytic degeneration with delayed differentiation, causing a reduction in the population of spermatogonia (Stra8 +) in the cellular proliferation (Pcna+) and disorganization of Vimentin filaments. These events had long-term repercussions on the quality of the seminiferous epithelium and semen. Our study demonstrates that at birth, there is a period that the testes are extremely sensitive to DEHP exposure, which leads to gonocytic degeneration and delay in their differentiation. This situation can have long-term repercussions or permanent effects on the quality of the seminiferous epithelium and sperm parameters.

中文翻译：

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新生儿接触邻苯二甲酸二（2-乙基己基）酯后性母细胞发育受到破坏

实验动物产前和/或产后服用邻苯二甲酸二（2-乙基己基）酯（DEHP）会导致精子发生的改变。然而，DEHP 影响生育能力的机制尚不清楚，可能是通过改变生殖细胞的存活和分化来实现的。本研究的目的是评估新生小鼠单次注射 DEHP 对生殖细胞增殖、分化和存活的影响及其对生精上皮和精子质量的长期影响。使用分为对照组和 DEHP 组的 BALB/c 小鼠。DEHP 组中的每只动物在出生时均接受 500 mg/Kg 的单剂量。在产后 1、2、4、6、8、10 和 70 天 (dpp) 对动物进行分析。对睾丸组织进行形态学分析，以确定不同类型的生殖细胞、分化指数、生精上皮改变以及对 Stra8、Pcna 和 Vimentin 蛋白的免疫反应性。在 70 dpp 时对生精上皮和精子质量进行长期评估。DEHP动物组呈现单核细胞变性并延迟分化，导致细胞增殖(Pcna+)中精原细胞(Stra8+)数量减少以及波形蛋白丝的解体。这些事件对生精上皮和精液的质量产生长期影响。我们的研究表明，出生时，睾丸有一段时期对 DEHP 暴露极其敏感，这会导致生殖细胞变性并延迟其分化。这种情况可能会对生精上皮的质量和精子参数产生长期影响或永久影响。