Fusarium wilt, caused by the fungus Fusarium oxysporum f. sp. phaseoli, is one of the most prevalent diseases in the common bean crop (Phaseolus vulgaris). However, there is little information regarding the genetics of reaction to this disease. Thus, the aims of this study were to estimate genetic parameters, identify quantitative trait loci (QTLs) associated with reaction to Fusarium wilt, and validate the markers identified. A total of 165 progenies of the population BRS FP403 × BRS Horizonte were evaluated. Evaluation was made in 2016 and 2017 in field trials in an area infested with the pathogen in Santo Antônio de Goiás, Brazil. Genotyping of single nucleotide polymorphism and SilicoDArT, where DArT is Diversity Arrays Technology, markers using the DArTseq technology was performed in the F2 generation. Analyses of the phenotypic data indicated the presence of variability among the progenies and the possibility of success from selection, with high estimates of heritability (90%) and gain from selection (37%). A genetic map was obtained with 702 markers and spanned 3069 cM. Five QTLs associated with reaction to Fusarium wilt were identified on five chromosomes. There was interaction between the QTLs and years. Through joint analysis, four QTLs were identified on chromosomes Pv01, Pv02, Pv03, and Pv04, explaining 5.8%–40.5% of the variation. The QTL FOP2.3403H explained 40.5% of phenotypic variation and was stable over the 2 years. From this QTL, two hydrolysis probes were developed for genotyping in a panel of lines for validation. Selection efficiency was over 92%, indicating considerable potential for use in marker‐assisted selection.

中文翻译：

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菜豆品种 BRS FP403 中与枯萎病反应相关的 QTL 鉴定和分子标记验证

镰刀菌枯萎病，由真菌引起尖孢镰刀菌F。sp。菜豆，是常见豆类作物中最常见的病害之一（菜豆）。然而，关于这种疾病的遗传反应的信息很少。因此，本研究的目的是估计遗传参数，鉴定与镰刀菌枯萎病反应相关的数量性状基因座（QTL），并验证所鉴定的标记。总共评估了 BRS FP403 × BRS Horizo​​nte 群体的 165 个后代。2016 年和 2017 年在巴西圣安东尼奥德戈亚斯州病原体感染地区进行了实地试验评估。单核苷酸多态性和 SilicoDArT 的基因分型，其中 DArT 是多样性阵列技术，使用 DArTseq 技术的标记在 F 中进行2一代。表型数据的分析表明后代之间存在变异性，并且选择成功的可能性，遗传力（90％）和选择增益（37％）的估计较高。获得了包含 702 个标记、跨度为 3069 cM 的遗传图谱。在 5 条染色体上鉴定出 5 个与镰刀菌枯萎病反应相关的 QTL。QTL与年份之间存在交互作用。通过联合分析，在染色体Pv01、Pv02、Pv03和Pv04上鉴定出4个QTL，解释了5.8%~40.5%的变异。QTL FOP2.3403H解释了 40.5% 的表型变异，并且在 2 年内保持稳定。根据该 QTL，开发了两个水解探针，用于在一组品系中进行基因分型以进行验证。选择效率超过 92%，表明在标记辅助选择中具有巨大的应用潜力。