The reproductive performance of chickens mainly depends on the development of follicles. Abnormal follicle development can lead to decreased reproductive performance and even ovarian disease among chickens. Chicken is the only non-human animal with a high incidence of spontaneous ovarian cancer. In recent years, the involvement of circRNAs in follicle development and atresia regulation has been confirmed. In the present study, we used healthy and atretic chicken follicles for circRNA RNC-seq. The results showed differential expression of circRALGPS2. It was then confirmed that circRALGPS2 can translate into a protein, named circRALGPS2-212aa, which has IRES activity. Next, we found that circRALGPS2-212aa promotes apoptosis and autophagy in chicken granulosa cells by forming a complex with PARP1 and HMGB1. Our results revealed that circRALGPS2 can regulate chicken granulosa cell apoptosis and autophagy through the circRALGPS2-212aa/PARP1/HMGB1 axis.

中文翻译：

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闭锁卵泡中高表达的circRALGPS2通过编码新蛋白诱导鸡颗粒细胞凋亡和自噬

鸡的繁殖性能主要取决于卵泡的发育情况。卵泡发育异常会导致鸡的繁殖性能下降，甚至卵巢疾病。鸡是唯一一种自发性卵巢癌高发的非人类动物。近年来，circRNA参与卵泡发育和闭锁调节已被证实。在本研究中，我们使用健康和闭锁鸡卵泡进行 circRNA RNC-seq。结果显示circRALGPS2的差异表达。随后证实circRALGPS2可以翻译成一种具有IRES活性的蛋白质，命名为circRALGPS2-212aa。接下来，我们发现circRALGPS2-212aa通过与PARP1和HMGB1形成复合物来促进鸡颗粒细胞的凋亡和自噬。我们的结果表明，circRALGPS2可以通过circRALGPS2-212aa/PARP1/HMGB1轴调节鸡颗粒细胞凋亡和自噬。