The numerous beneficial probiotic properties of Lactobacillus reuteri (L. reuteri) include decreasing metabolic syndrome, preventing disorders linked to oxidative stress, improving gut flora imbalances, controlling immunological function, and extending life span. Exposure to ionizing radiation is closely associated with several disorders. We examined the protective and salvaging effects of L. reuteri on ionizing radiation-induced injury to the intestinal tract, reproductive system, and nervous system of Drosophila melanogaster. We also examined its effects on lifespan, antioxidant capacity, progeny development, and behavioral aspects to assess the interaction between L. reuteri and ionizing radiation-induced injury. The findings demonstrated that L. reuteri improved the median survival time following irradiation and greatly extended its lifespan. In addition, it raised SOD activity, reduced ROS levels in intestinal epithelial cells, and increased the quantity of intestinal stem cells. Furthermore, L. reuteri enhanced the adult male flies’ capacity to move. It also successfully safeguarded the generations’ growth and development. L. reuteri dramatically enhanced expression of the AMPKα gene and regulated expression of its pathway-related gene, mTOR, as well as the autophagy-related genes Atg1 and Atg5 in female Drosophila exposed to irradiation. Notably, no prior reports have been made on the possible effects of L. reuteri on injuries caused by irradiation. As a result, our research offers important new information regarding L. reuteri's possible role as a shield against ionizing radiation-induced injury.

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罗伊氏乳杆菌在减轻果蝇电离辐射损伤中的多方面作用

罗伊氏乳杆菌( L. reuteri ) 具有众多有益的益生菌特性，包括减少代谢综合征、预防与氧化应激相关的疾病、改善肠道菌群失衡、控制免疫功能和延长寿命。接触电离辐射与多种疾病密切相关。我们研究了罗伊氏乳杆菌对电离辐射引起的果蝇肠道、生殖系统和神经系统损伤的保护和挽救作用。我们还检查了它对寿命、抗氧化能力、后代发育和行为方面的影响，以评估罗伊氏乳杆菌和电离辐射引起的损伤之间的相互作用。研究结果表明，罗伊氏乳杆菌提高了辐射后的中位生存时间，并大大延长了其寿命。此外，它还能提高SOD活性，降低肠上皮细胞中ROS水平，并增加肠干细胞的数量。此外，罗伊氏乳杆菌增强了成年雄性果蝇的移动能力。也成功保障了几代人的成长和发展。在受到辐射的雌性果蝇中，罗伊氏乳杆菌显着增强了 AMPKα 基因的表达，并调节了其通路相关基因 mTOR 以及自噬相关基因 Atg1 和 Atg5 的表达。值得注意的是，之前没有关于罗伊氏乳杆菌对辐射造成的损伤可能产生的影响的报道。因此，我们的研究提供了关于罗伊氏乳杆菌作为抵御电离辐射引起的损伤的屏障的可能作用的重要新信息。