Between 2016 and 2023, a cross-sectional study was conducted in the central region of Portugal in order to better understand the epidemiology and public health risks resulting from the handling and consumption of game animals infected with Brucella spp. The seroprevalence and risk factors for Brucella spp. seropositivity were evaluated. Antibodies against Brucella spp. were determined using a commercial enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions. Results showed that in the 650 serum samples collected from red deer (n = 298) and wild boars (n = 352) in Portugal, 21.7% (n = 141; 95% CI: 18.6–25.1%) tested positive. Wild boar had a significantly higher prevalence (35.5%; 95% CI: 30.5–40.8%) than red deer (5.4%, 95% CI: 3.1–8.6%; p ≤ 0.001). Risk factors for seropositivity were investigated using multivariable logistic regression models. The odds of being seropositive was 8.39 (95% CI: 4.75–14.84; p ≤ 0.001) times higher in wild boar than in red deer. Correlations between sex, age, body condition, and seropositivity could not be observed. The higher seroprevalence in wild boar suggests that this species may primarily contribute to the Brucella spp. ecology in central Portugal.

中文翻译：

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寻找答案：评估布鲁氏菌属。葡萄牙中部红鹿和野猪的血清阳性率和风险

2016 年至 2023 年间，在葡萄牙中部地区进行了一项横断面研究，以便更好地了解因处理和食用感染布鲁氏菌属的野味动物而造成的流行病学和公共卫生风险。布鲁氏菌属的血清流行率和危险因素。评估血清阳性。布鲁氏菌抗体。根据制造商的说明，使用商业酶联免疫吸附测定（ELISA）测定。结果显示，在葡萄牙采集的马鹿 (n = 298) 和野猪 (n = 352) 的 650 份血清样本中，21.7% (n = 141；95% CI: 18.6–25.1%) 检测呈阳性。野猪的患病率（35.5%；95% CI：30.5–40.8%）显着高于马鹿（5.4%，95% CI：3.1–8.6%；p ≤ 0.001）。使用多变量逻辑回归模型研究血清阳性的危险因素。野猪血清呈阳性的几率是马鹿的 8.39 倍（95% CI：4.75–14.84；p ≤ 0.001）。无法观察到性别、年龄、身体状况和血清阳性之间的相关性。野猪中较高的血清阳性率表明该物种可能主要导致布鲁氏菌属。葡萄牙中部的生态。