Utilizing a gene evolution-oriented approach for gene cluster mining, a cryptic cytochalasin-like gene cluster (sla) in Antarctic-derived Simplicillium lamelliciola HDN13430 was identified. Compared with the canonical cytochalasin biosynthetic gene clusters (BGCs), the sla gene cluster lacks the key α,β-hydrolase gene. Heterologous expression of the sla gene cluster led to the discovery of a new compound, slamysin (1), characterized by an N-acylated amino acid structure and demonstrating weak anti-Bacillus cereus activity. These findings underscore the potential of genetic evolution in uncovering novel compounds and indicating specific adaptive evolution within specialized habitats.

中文翻译：

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基于进化的从 Simplicillium lamelliciola HDN13430 细胞松弛素样基因簇中发现聚酮化合物酰化缬氨酸

利用面向基因进化的基因簇挖掘方法，在南极衍生的Simplicillium lamelliciola HDN13430 中鉴定出一个隐秘的细胞松弛素样基因簇 ( sla )。与典型的细胞松弛素生物合成基因簇（BGC）相比，sla基因簇缺少关键的α,β-水解酶基因。sla基因簇的异源表达导致了一种新化合物slamysin ( 1 )的发现，其特征在于N-酰化氨基酸结构并表现出弱的抗蜡样芽孢杆菌活性。这些发现强调了遗传进化在发现新化合物和表明特定栖息地内的特定适应性进化方面的潜力。