A new polyol polyketide, named retinestatin (1), was obtained and characterized from the culture of a Streptomyces strain, which was isolated from a subterranean nest of the termite Reticulitermes speratus kyushuensis Morimoto. The planar structure of 1 was elucidated on the basis of the cumulative analysis of ultraviolet, infrared, mass spectrometry, and nuclear magnetic resonance spectroscopic data. The absolute configuration of 1 at 12 chiral centers was successfully assigned by employing a J-based configuration analysis in combination with ROESY correlations, a quantum mechanics-based computational approach to calculate NMR chemical shifts, and a 3 min flash esterification by Mosher’s reagents followed by NMR analysis. Biological evaluation of retinestatin (1) using an in vitro model of Parkinson’s disease revealed that 1 protected SH-SY5Y dopaminergic cells from MPP⁺-induced cytotoxicity, indicating its neuroprotective effects.

中文翻译：

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Retinestatin，一种来自白蚁巢源链霉菌属的多元醇聚酮化合物。

一种新的多元醇聚酮化合物，命名为retinestatin ( 1 )，是从链霉菌菌株的培养物中获得并表征的，该菌株是从白蚁Reticulitermes speratus kyushuensis Morimoto的地下巢中分离出来的。根据紫外、红外、质谱和核磁共振波谱数据的累积分析，阐明了1的平面结构。通过采用基于J的构型分析结合 ROESY 相关性、基于量子力学的计算方法来计算 NMR 化学位移，以及通过 Mosher 试剂进行 3 分钟的快速酯化，然后使用核磁共振分析。使用帕金森病体外模型对Retinestatin ( 1 )进行生物学评估，结果显示1可以保护 SH-SY5Y 多巴胺能细胞免受 MPP ⁺诱导的细胞毒性，表明其具有神经保护作用。