The plasticity of the lipid profile of soybean oil has made the soybean a crop with value in feed, food and industrial uses. Soybean oil typically consists of approximately 10% of the fully saturated palmitic acid, which has value for industrial purposes, however it is generally undesirable in food applications. Two soybean KASII (ketoacyl‐ACP synthase II) genes have been previously implicated in the control of seed palmitic acid levels. Using forward genetics, we isolated four novel alleles in the GmKASIIB gene that result in increased levels of palmitic acid, ranging from 13% to 16% of the total fatty acid content, over multiple seasons in the field. Three of the KASIIB mutations result in independent amino acid substitutions at conserved positions in the KASII protein, and one single nucleotide polymorphism is likely to cause aberrant splicing of the KASIIB transcript. These new mutations can be used in breeding applications to increase palmitic acid content and to probe the control of fatty acid biosynthesis in soybean seeds.

中文翻译：

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KASIIB 突变导致大豆种子中棕榈酸含量增加

大豆油脂质的可塑性使大豆成为一种具有饲料、食品和工业用途价值的作物。大豆油通常含有约 10% 的完全饱和棕榈酸，其具有工业用途价值，但在食品应用中通常不受欢迎。黄豆两颗卡斯II（酮酰基-ACP 合酶 II）基因先前已被认为与种子棕榈酸水平的控制有关。利用正向遗传学，我们在甲基化KASIIB该基因导致田间多个季节棕榈酸含量增加，占总脂肪酸含量的 13% 至 16%。其中三个韩国航空工业协会突变导致 KASII 蛋白保守位置上的独立氨基酸取代，并且一种单核苷酸多态性可能导致该蛋白的剪接异常。韩国航空工业协会成绩单。这些新突变可用于育种应用，以增加棕榈酸含量并探索大豆种子中脂肪酸生物合成的控制。