Despite the worldwide occurrence of bartonellae in a broad range of mammal species, in which they usually cause a long-lasting erythrocytic bacteremia, few studies reported Bartonella spp. in avian hosts. The present work aimed to investigate the occurrence and molecular identity of Bartonella spp. infecting birds in the Pantanal wetland, central-western Brazil using a multigene approach. For this purpose, blood samples were collected from 517 individuals from 13 avian orders in the states of Mato Grosso and Mato Groso do Sul. DNA was extracted from avian blood and 500/517 (96.7%) samples were positive in a conventional PCR targeting the avian β-actin gene. Nineteen (3.8%) out of 500 avian blood samples were positive in a qPCR assay for Bartonella spp. based on the nuoG gene. Among 19 avian blood DNA samples positive in the qPCR for Bartonella spp., 12 were also positive in the qPCR for Bartonella based on the 16S-23S RNA Intergenic region (ITS). In the PCR assays performed for molecular characterization, one 16S rRNA, three ribC, and one nuoG sequences were obtained. Based on BLASTn results, while 1 nuoG, 2 ribC, and 2 ITS sequences showed high identity to Bartonella henselae, one 16S rRNA and 2 ITS showed high similarity to Bartonella machadoae in the sampled birds. Bartonella spp. related to B. henselae and B. machadoae were detected, for the first time, in wild birds from the Brazilian Pantanal.

尽管巴尔通体在世界范围内广泛存在于多种哺乳动物中，并且通常会引起持久的红细胞菌血症，但很少有研究报道巴尔通体属。在鸟类宿主中。目前的工作旨在研究巴尔通体属的发生和分子身份。使用多基因方法感染巴西中西部潘塔纳尔湿地的鸟类。为此，我们从马托格罗索州和南马托格罗索州 13 个禽类的 517 个人身上采集了血液样本。从禽类血液中提取 DNA，在针对禽类 β-肌动蛋白基因的常规 PCR 中，500/517 (96.7%) 的样本呈阳性。500 份禽类血液样本中有 19 份 (3.8%) 在 qPCR 检测中巴尔通体属呈阳性。基于nuo G 基因。在 19 份巴尔通体qPCR 检测呈阳性的禽类血液 DNA 样本中，有 12 份基于 16S-23S RNA 基因间区域 (ITS) 的巴尔通体qPCR 检测也呈阳性。在进行分子表征的 PCR 测定中，获得了1 个 16S rRNA、3 个rib C 和 1 个nuoG序列。根据 BLASTn 结果，1 个nuoG、 2 个ribC和 2 个 ITS 序列与采样鸟类中的巴尔通体表现出高度同一性，而1 个 16S rRNA 和 2 个 ITS 与马查多巴尔通体表现出高度相似性。巴尔通体属 首次在巴西潘塔纳尔湿地的野生鸟类中检测到与B. henselae和B. machadoae相关的细菌。